Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E. coli Proteoform Families and G-PTM-D Database Expansion

ABSTRACT: E. coli (KL334) lysate data of a) intact-mass proteomics using NeuCode-labeled SILAC across 3 biological replicates, 10 molecular weight fractions, and 2 technical replicates, for elucidating proteoform families; b) bottom-up proteomics using label-free culture across 10 molecular weight fractions and 2 technical replicates, for constructing G-PTM-D augmented database.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: Lloyd M. Smith

PROVIDER: MSV000081144 | MassIVE | Tue Jun 06 17:39:00 BST 2017

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

undefined

Project description:Ambr 2 - Three technical replicates

| PRJEB51873 | ENA

Constructing Human Proteoform Families Using Intact-Mass and Top-Down Proteomics with a Multi-Protease G-PTM-D Database

Project description:This data set contains the following: 1) 66 raw files for NeuCode-labeled human proteoforms from the Jurkat cell line analyzed via "intact-mass" (i.e., LC-MS, with no precursor fragmentation). Three biological replicates were performed (files from the different replicates are labeled with 022317, 031617, or 031817, respectively). For each replicate, proteoforms were separated offline using a 12% Tris-acetate Gelfree cartridge and 11 fractions were collected. Two technical replicate LC-MS injections of each fraction were performed, yielding a total of 66 raw files (3 biological replicates x 11 fractions x 2 injections). 2) 22 raw files for label-free human proteoforms from the Jurkat cell line analyzed via "top-down" (i.e., LC-MS/MS, with precursor fragmentation). One biological replicate was performed (labeled 032017). Proteoforms were separated offline using a 12% Tris-acetate Gelfree cartridge and 11 fractions were collected. Two technical replicate LC-MS/MS injections of each fraction were performed, yielding a total of 22 raw files (1 biological replicate x 11 fractions x 2 injections). 3) Multi-protease and trypsin-only pruned G-PTM-D databases used for proteoform identification. Note that the bottom-up data used to generate these databases can be found elsewhere on MassIVE (MSV000083304).

2019-05-13 | MSV000083768 | MassIVE

Expanding Proteoform Identifications in Top-Down Proteomic Analyses by Constructing Proteoform Families

Project description:Raw top-down LC-MS/MS files from 12% GELFREE separated fractions of yeast. Raw files for 2 technical replicates of each fraction, in total 24 raw files.

2017-10-09 | MSV000081592 | MassIVE

ENCODE Transcript Mapping for Human Neutrophil (PMN) Total RNA

Project description:Transcript Mapping on Affymetrix ENCODE arrays for 10 different biological replicates of Neutrophil (PMN) Total RNA (each with 2 technical replicates). Each biological sample is from a different individual. Keywords = Transcript Mapping, Human, Affymetrix, Genome Tiling Arrays Keywords: other

2005-05-19 | GSE2678 | GEO

Sparus aurata

Project description:Technical replicates to determine array-to-array reproducibility.

| PRJNA113521 | ENA

Proteomic characteristics of hepatopancreas in cold-tolerant families and common families of Litopenaeus vannamei

Project description:In this study, hepatopancreas tissues of cold-tolerant and common families of Litopenaeus vannamei were selected by the Guangxi Academy of Fishery Sciences for proteomic study. Hepatopancreas were treated at 28℃, 18℃ and 10℃ respectively based on DIA technology. There were three replicates of cold-tolerant families and common families at 28℃, 18℃ and 10℃ respectively, and each replicate contained hepatopancreas mixed samples of 6 shrimp at random. A total of 18 samples were analyzed. The original data of common families at 28℃, 18℃ and 10℃ were named Lv-C-28、Lv-C-18 and Lv-C-10 respectively, while the original data of cold-tolerant families at 28℃, 18℃ and 10℃ were named Lv-T-28、Lv-T-18 and Lv-T-10 respectively.

2023-06-09 | PXD041965 |

e1cf8fab-85c7-426e-b7fe-c57f9805907e - samples

Project description:Bacteriophage Immunoprecipitation Sequencing (PhIP-Seq) datasets of three eHHV-6B-positive SLE patients (three technical replicates), six eHHV-6B-negative SLE patients (three technical replicates), and a negative control lacking sera or plasma (12 technical replicates; Illumina NextSeq) and sequencing data of HHV-6 peptide phage library (two technical replicates; Illumina NextSeq).

| EGAD00001015429 | EGA

PhIP-seq of Japanese patients with SLE

| EGAS00001007955 | EGA

Homo sapiens

Project description:DNA methylation profiles of liver and placenta technical replicates

| PRJNA229912 | ENA

Measurement of inter-individual variability in gene expression in mouse neutrophils

Project description:We extracted RNA from C57BL/6 mouse neutrophils to measure inter-individual variability in gene expression. 5 of the 10 blood samples were analyzed separately, the other 5 were pooled then split into 5 technical replicates to measure technical variability. We could then measure inter-individual variability in gene expression and compare it to the amplitude of miRNA-guided repression by miR-223.

2016-09-21 | GSE59549 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data