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PPARG interactions with UBR5 and MRE11-RAD50-NBS1 are required for DNA damage response and repair.

ABSTRACT: PPARG interactome datasets containing (1) Flag-PPARG AP-MS, identifying MRE11, RAD50 and NBS1 as novel interactors, (2) tandem-IP (in solution and silver fragments) of PPARG-Strep and Flag-NBS1, which identifies PPARG interaction with UBR5 (3) tandem-IP (same as 2) with BS3 cross-linking illustrates the binding interface between PPARG and NBS1. These mass spec data and other biochemical data demonstrate PPARG novel roles in promoting DNA damage response and repair, by activating the ATM pathway.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Marlene Rabinovitch

PROVIDER: MSV000083257 | MassIVE | Tue Dec 18 11:46:00 GMT 2018

REPOSITORIES: MassIVE

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