Proteomics

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Analysis of mitochondrial N termini by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)

ABSTRACT: Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samples. Identification of distinct N-terminal profiles in mitochondrial-enriched B-ALL and AML samples as one of the applications of HUNTER. Mitochondria are enriched from 2.5 million cultured B-ALL or primary patient AML cells using pressure cycling (PCT) assisted lysis and differential density centrifugation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Philipp Lange   Dr. Pitter F. Huesgen  

PROVIDER: MSV000083504 | MassIVE | Thu Feb 28 17:57:00 GMT 2019

SECONDARY ACCESSION(S): PXD012919

REPOSITORIES: MassIVE

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Publications

Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples.

Weng Samuel S H SSH   Demir Fatih F   Ergin Enes K EK   Dirnberger Sabrina S   Uzozie Anuli A   Tuscher Domenic D   Nierves Lorenz L   Tsui Janice J   Huesgen Pitter F PF   Lange Philipp F PF  

Molecular & cellular proteomics : MCP 20190830 11

Protein N termini unambiguously identify truncated, alternatively translated or modified proteoforms with distinct functions and reveal perturbations in disease. Selective enrichment of N-terminal peptides is necessary to achieve proteome-wide coverage for unbiased identification of site-specific regulatory proteolytic processing and protease substrates. However, many proteolytic processes are strictly confined in time and space and therefore can only be analyzed in minute samples that provide i  ...[more]

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