Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Glomerular tissue extra-cellular proteome in focal segemental glomerular sclerosis

ABSTRACT: Glomeruli were isolated from formalin-fixed paraffin embedded (FFPE) preserved human kidney biopsies using laser capture micro-dissection. Isolated tissue was de-cellularized by extraction using an ammonium hydroxide/NP-40 protocol. The residual extra-cellular fraction was analyzed by 1-dimensional C18 RP LCMS.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael L. Merchant, PhD

PROVIDER: MSV000084083 | MassIVE | Fri Jul 12 11:55:00 BST 2019

SECONDARY ACCESSION(S): PXD014594

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Podocyte-specific deletion of tubular sclerosis complex 2 causes focal segmental glomerular sclerosis and progressive renal failure

Project description:Obesity can initiate and accelerate the progression of kidney diseases. However, it remains unclear how obesity affects renal dysfunction. Here, we show that a newly generated podocyte-specific tubular sclerosis complex 2 (Tsc2) knockout mouse model (Tsc2∆podocyte) develops proteinuria and dies due to end-stage renal dysfunction by 10 weeks of age. Tsc2∆podocyte mice exhibit an increased glomerular size and focal segmental glomerulosclerosis, including podocyte foot process effacement, mesangial sclerosis and proteinaceous casts. Podocytes isolated from Tsc2∆podocyte mice show nuclear factor, erythroid derived 2, like 2-mediated increased oxidative stress response on microarray analysis and their autophagic activity is lowered through the mammalian target of rapamycin (mTOR)—unc-51-like kinase 1 pathway. Rapamycin attenuated podocyte dysfunction and extends survival in Tsc2∆podocyte mice. Additionally, mTOR complex 1 (mTORC1) activity is increased in podocytes of renal biopsy specimens obtained from obese patients with chronic kidney disease. Our work shows that mTORC1 hyperactivation in podocytes leads to severe renal dysfunction and that inhibition of mTORC1 activity in podocytes could be a key therapeutic target for obesity-related kidney diseases.

2020-03-21 | GSE106828 | GEO

Focal segmental glomerular sclerosis urine degradome pilot study

Project description:Pilot urine peptidome study to evaluate Proteasix tool to predict proteolytic activity associated with nephrotic syndrome disease.

2019-07-12 | MSV000084084 | MassIVE

microRNA expression in vulvar melanoma

Project description:microRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from vulvar melanoma compared to primary cutaneous melanoma

2022-07-16 | GSE208174 | GEO

mRNA expression in vulvar melanoma

Project description:mRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from vulvar melanoma compared to primary cutaneous melanoma.

2022-07-16 | GSE208172 | GEO

microRNA expression in vaginal melanoma

Project description:microRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from vaginal melanoma compared to adjacent healthy vaginal mucosa.

2022-07-16 | GSE208175 | GEO

mRNA expression in vaginal melanoma

Project description:mRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from vaginal melanoma compared to adjacent healthy vaginal mucosa.

2022-07-16 | GSE208173 | GEO

mRNA expression in ulcerated primary cutaneous melanoma (validation cohort)

Project description:mRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from ulcerated primary cutaneous melanoma compared to non-ulcerated primary cutaneous melanoma

2022-08-13 | GSE211093 | GEO

mRNA expression in ulcerated primary cutaneous melanoma

Project description:mRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from ulcerated primary cutaneous melanoma compared to non-ulcerated primary cutaneous melanoma

2022-08-13 | GSE211091 | GEO

microRNA expression in ulcerated primary cutaneous melanoma

Project description:microRNA expression in RNA isolated from formalin fixed, paraffin embedded tissue from ulcerated primary cutaneous melanoma compared to non-ulcerated primary cutaneous melanoma

2022-08-13 | GSE211090 | GEO