Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Col4a1 mouse glomerular datasets

ABSTRACT: Glomeruli were isolated from Col4a1+/SVC and Ctrl mice. Samples were enriched for extracellular matrix. Glomerular extracellular matrix and cellular fractions were analysed by mass spectrometry.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Franziska Lausecker

LAB HEAD: Rachel Lennon

PROVIDER: PXD022363 | Pride | 2022-08-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20150128_MR1.raw	Raw
	20150128_MR10.raw	Raw
	20150128_MR11.raw	Raw
	20150128_MR12.raw	Raw
	20150128_MR2.raw	Raw

Items per page:

1 - 5 of 26

Publications

Identification of an Altered Matrix Signature in Kidney Aging and Disease.

Randles Michael J MJ Lausecker Franziska F Kong Qingyang Q Suleiman Hani H Reid Graeme G Kolatsi-Joannou Maria M Davenport Bernard B Tian Pinyuan P Falcone Sara S Falcone Sara S Potter Paul P Van Agtmael Tom T Norman Jill T JT Long David A DA Humphries Martin J MJ Miner Jeffrey H JH Lennon Rachel R

Journal of the American Society of Nephrology : JASN 20210528 7

<h4>Background</h4>Accumulation of extracellular matrix in organs and tissues is a feature of both aging and disease. In the kidney, glomerulosclerosis and tubulointerstitial fibrosis accompany the decline in function, which current therapies cannot address, leading to organ failure. Although histologic and ultrastructural patterns of excess matrix form the basis of human disease classifications, a comprehensive molecular resolution of abnormal matrix is lacking.<h4>Methods</h4>Using mass spectr ...[more]

PMID: 34049963

Similar Datasets

Col4a3 mouse glomerular datasets

Project description:Glomeruli were isolated from Col4a3-/- and Ctrl mice. The extracellular matrix was enriched and both cellular and matrix fraction were analysed by mass spectrometry.

2022-08-12 | PXD022392 | Pride

Col4a5 mouse glomerular extracellular matrix

Project description:Alport mouse model (Col4a5) have been submitted for proteomic analysis. Glomeruli have been isolated from mouse kidneys, extracellular matrix has been enriched and analysed by mass spectrometry.

2022-08-12 | PXD022227 | Pride

Human kidney proteomics, glomerular and tubular cellular and matrix fractions

Project description:Glomerular and tubulointerstitial fractions were enriched from human kidneys samples. Ages of human kidney samples: 15, 29, 37, 61, 67 and 69 years of age. The glomerular and tubulointerstitial samples were processed to enrich for extracellular matrix components and the fractions analysed by mass spectrometry.

2022-02-22 | PXD022219 | Pride

Mouse kidney glomerular extracellular matrix LC-MSMS

Project description:Across the spectrum of human disease there are significant changes in the composition and organisation of extracellular matrix (ECM). Furthermore, disease severity can vary according to race and sex, yet mechanisms for these variations are unclear. Using the kidney glomerulus as a model system, we tested the hypothesis that genetic background and sex influence composition and organisation of ECM. We characterised ECM from healthy mice with different predisposition to glomerular disease using global microarray, discovery proteomics and electron microscopy. Transcriptomic and proteomic analyses revealed unique strain and sex dependent glomerular ECM signatures with novel ECM proteins, which relate to glomerular dysfunction with leakage of macromolecules into the glomerular filtrate. In addition, we found striking structural changes in basement membranes in disease-susceptible mice. These findings were not explained by mutations in known ECM or glomerular genes. Pathway analysis of merged transcriptomic and proteomic datasets identified potential ECM regulatory pathways involving inhibition of matrix metalloproteinases, LXR/RXR, NRF2, notch and CDK5. These pathways may therefore alter ECM and confer susceptibility to disease.

2016-07-09 | PXD000811 | Pride

Integrin adhesion complexes formed on different basement membrane ligands

Project description:Adhesion complexes isolated from cells in culture. Podocytes were attached to different extracellular matrix ligands and adhesion complex isolated.

2020-03-09 | PXD017913 | Pride

Human kidney proteomics, glomerular and tubular cellular and matrix fractions

Project description:kidney samples: 15, 29, 37, 61, 67 and 69 years of age. The glomerular and tubulointerstitial samples were processed to enrich for extracellular matrix components and the fractions analysed by mass spectrometry.

2021-10-13 | PXD026002 | Pride

Proteomic analysis of glomerular organoid extracellular matrix

Project description:Introduction Points to consider adding: -The matrix in the kidney/glomerulus is specialised and complex with basement membrane structures in addition to interstitial matrix components. -The glomerular basement membrane undergoes change in isoform composition of key matrix components including type IV collagen and laminin. -The matrix composition of kidney organoids has not been yet been investigated in a global manner.

2019-01-03 | PXD012198 | Pride

Isocitrate dehydrogenase 1 mutations prime the all-trans-retinoic acid myeloid differentiation pathway in acute myeloid leukemia HL-60 cells

Project description:Human acute myeloid leukemia HL60 cells were transduced with IDH1-WT or IDH1-R132H, and corresponding sub-clones were generated by FACS. In a first series of experiments, cells expressing wt and mutant IDH1were treated with either 1ÂµM all-trans retinoic acid (ATRA) or with vehicle (DMSO) for 24 hrs, resulting in 4 groups of samples (WT-CTRL, WT-ATRA, Mutant-CTRL, Mutant-ATRA). In a second series of assays, WT-IDH1 cells were pre-treated for 2 days with either 100 ÂµM octyl ester of (R)-2-hydroxyglutarate ((R)-2-HG) or 1-octanol (octyl), and then treated with 1 ÂµM ATRA or vehicle for 24 hrs, again resulting in 4 groups of samples (Octyl-CTRL, Octyl-ATRA, 2HG-CTRL, 2HG-ATRA). All assays were performed in triplicate (4 replicates for Mutant-CTRL and Mutant-ATRA).

2017-03-01 | E-MTAB-4327 | biostudies-arrayexpress

Gene expression profiling of retrovirus PDGFB-induced glioma

Project description:We performed gene expression profilings of platelet-derived growth factor subunit B (PDGFB)-induced mouse glioma to compare the differential transcriptome profiles between Ctrl-T tumor cells and Olig2cKO tumor cells. Expression profiling of Ctrl-T and Olig2cKO brain tumor (glioma) cells, normal oligodendrocyte progenitor cells (OPCs), normal astrocytes, and normal brain cortex by high-throughput sequencing.

2016-05-17 | E-GEOD-80087 | biostudies-arrayexpress

Visceral Endoderm differentiation mediated by Hnf1B and is required for epiblast survival

Project description:E5.0 entails an intermediate state for primitive endoderm (PE) to visceral endoderm (VE) differentiation: a process required for proper embryonic development. Hnf1b is essential for primitive to visceral endoderm differentiation from E4.5 to E5.5. To understand development at E5.0 and pinpoint the role Hnf1b in proper embryonic development, embryos were harvest at E5.0 and single cells from three WT embryos and 3 Hnf1b KO embryos were isolated, dissociated, and scRNA sequenced using smartseq2. For experiments on WT embryos, CD1 females were crossed with CD1 or homozygous E-cadherin-GFP males. Hnf1b -/+22 line was derived through IVF of oocytes of Black6 mice with EMMA strain EM:07827(B6.129P2(D2)-Hnf1btm1Sce/Orl) Sperm, that was bought from the EMMA institution in Orleans.

2024-11-19 | E-MTAB-11140 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data