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High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip

ABSTRACT: Development and evaluation of nested nanowell (N2) chip in single-cell proteomics. The newly designed chip helps to improve the sensitivity and robustness of the tandem mass tag labeling approach by sizing down wells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Ying Zhu

PROVIDER: MSV000086809 | MassIVE | Thu Feb 04 17:27:00 GMT 2021

REPOSITORIES: MassIVE

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High-throughput and high-efficiency sample preparation for single-cell proteomics using a nested nanowell chip.

Woo Jongmin J Williams Sarah M SM Markillie Lye Meng LM Feng Song S Tsai Chia-Feng CF Aguilera-Vazquez Victor V Sontag Ryan L RL Moore Ronald J RJ Hu Dehong D Mehta Hardeep S HS Cantlon-Bruce Joshua J Liu Tao T Adkins Joshua N JN Smith Richard D RD Clair Geremy C GC Pasa-Tolic Ljiljana L Zhu Ying Y

Nature communications 20211029 1

Global quantification of protein abundances in single cells could provide direct information on cellular phenotypes and complement transcriptomics measurements. However, single-cell proteomics is still immature and confronts many technical challenges. Herein we describe a nested nanoPOTS (N2) chip to improve protein recovery, operation robustness, and processing throughput for isobaric-labeling-based scProteomics workflow. The N2 chip reduces reaction volume to <30 nL and increases capacity to > ...[more]

PMID: 34716329

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