APEX2-MLF2 & d133 ORF10 IP (Table S1, Prophet et al.)
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ABSTRACT: Datasets generated using WT or TorsinKO HeLa cells expressing APEX2-MLF2-HA or d133 ORF10 from the KSHV. Data were used to generate Table S1.
Project description:MLF2-APEX2 fusion-based proximity labeling of nuclear envelope bleb composition. APEX reactions were conducted in WT and TorsinKO HeLa cells.
Project description:The truncated d133-ORF10-FLAG from KSHV was expressed and immunoprecipitated from WT and TorsinKO HeLa cells. The datasets were compared to an untransfected control. As d133-ORF10 localizes to blebs in TorsinKO cells, this approach allows probing of bleb protein contents.
Project description:HSPA1A was immunoprecipitated from WT or TorsinKO HeLa cells. A band around 27 kDa was uniquely found to co-immunoprecipitate with HSPA1A in TorsinKO cells. To identify this band, it was extracted and subjected to mass spectrometry.
Project description:HSPA1A was immunoprecipitated from WT or TorsinKO HeLa cells. A band around 27 kDa was uniquely found to co-immunoprecipitate with HSPA1A in TorsinKO cells. To identify this band, it was extracted and subjected to mass spectrometry.
Project description:MLF2 was purified from mammalian Expi293 cells as a fusion with MBP. As MLF2 associates tightly with HSP70/HSPA1A, this resulted in a stoichiometric co-purification with HSP70 members. To identify which ones, we subjected the whole purification or a gel-extracted band around 70 kDa to mass spectrometry.
Project description:MLF2 was purified from mammalian Expi293 cells as a fusion with MBP. As MLF2 associates tightly with HSP70/HSPA1A, this resulted in a stoichiometric co-purification with HSP70 members. To identify which ones, we subjected the whole purification or a gel-extracted band around 70 kDa to mass spectrometry.