Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

APEX2-MLF2 (Table S1, Prophet et al.)

ABSTRACT: MLF2-APEX2 fusion-based proximity labeling of nuclear envelope bleb composition. APEX reactions were conducted in WT and TorsinKO HeLa cells.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Christian Schlieker

PROVIDER: MSV000090177 | MassIVE | Mon Aug 22 13:30:00 BST 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

APEX2-MLF2 & d133 ORF10 IP (Table S1, Prophet et al.)

Project description:Datasets generated using WT or TorsinKO HeLa cells expressing APEX2-MLF2-HA or d133 ORF10 from the KSHV. Data were used to generate Table S1.

2022-07-07 | MSV000089840 | MassIVE

Proximity labeling via MLF2-APEX2 fusion

Project description:Proximity labeling approach to identify protein inside nuclear envelope blebs arising in Torsin-deficient HeLa cells. WT and TorsinKO cells were engineered to express MLF2-APEX2 fusion. APEX reaction was carried out via 1 mM H2O2 for 1 minute in the presence of biotin phenol. Biotinylated protein were captured via streptavidin beads. Each APEX reaction was accompanied by an untreated (no H2O2) control.

2022-08-25 | PXD036262 | Pride

d133 ORF10 IP (Table S2, Prophet et al.)

Project description:The truncated d133-ORF10-FLAG from KSHV was expressed and immunoprecipitated from WT and TorsinKO HeLa cells. The datasets were compared to an untransfected control. As d133-ORF10 localizes to blebs in TorsinKO cells, this approach allows probing of bleb protein contents.

2022-08-23 | MSV000090186 | MassIVE

20-37 kDa band of HSPA1A IP (Table S3, Prophet et al.)

Project description:HSPA1A was immunoprecipitated from WT or TorsinKO HeLa cells. A band around 27 kDa was uniquely found to co-immunoprecipitate with HSPA1A in TorsinKO cells. To identify this band, it was extracted and subjected to mass spectrometry.

2022-08-23 | MSV000090187 | MassIVE

GNPS - 20-37 kDa band of HSPA1A IP (Table S3, Prophet et al.)

2022-08-23 | MSV000090187 | GNPS

MLF2-MBP purification (Table S4, Prophet et al.)

Project description:MLF2 was purified from mammalian Expi293 cells as a fusion with MBP. As MLF2 associates tightly with HSP70/HSPA1A, this resulted in a stoichiometric co-purification with HSP70 members. To identify which ones, we subjected the whole purification or a gel-extracted band around 70 kDa to mass spectrometry.

2022-08-23 | MSV000090188 | MassIVE

GNPS - MLF2-MBP purification (Table S4, Prophet et al.)

2022-08-23 | MSV000090188 | GNPS

Nano-structural analysis of engrafted human induced pluripotent stem cell-derived cardiomyocytes in mouse hearts using a genetic-probe APEX2.

Project description:Using APEX2, an engineered peroxidase, as an imaging tag, we successfully located iPSC-derived cardiomyocytes (iPSC-CMs) engrafted in post-myocardial infarction mouse hearts for more than 6 months. APEX2 caused clear contrast in X-ray microscopy and electron microscopy, and relatively well organized sarcomere structures were formed in iPSC-CMs. Electron microscopic tomography further found the development of T-tubules and dyads in APEX2-labelled cells, which are the signature characteristics of the maturation of cardiomyocytes.

2018-12-01 | GSE99470 | GEO