Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HHIP protein interactions in lung cells provide insight into COPD pathogenesis

ABSTRACT: Affinity purification mass spectrometry (AP-MS) was performed to identify protein interactions with HHIP, a well-established COPD GWAS gene. HA-immunoprecipitations (n = 3) were conducted using IMR90 and 16HBE cell lines stably expressing HA-tagged HHIP.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr. Edwin K. Silverman Dr. Wenyi Wei

PROVIDER: MSV000096594 | MassIVE | Wed Dec 04 13:02:00 GMT 2024

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Identification of the BRD1 interactome and its impact on schizophrenia risk

Project description:We identified protein-protein interactions and chromatin binding sites for two isforms of BRD1 (BRD1-S and BRD1-L) using Co-IP MS/MS and ChIP-seq. BRD1 isoforms were cloned, epitope-tagged (pcDNA 6 V5-His6, Lifetechnologies) and stably expressed in HEK293T cells. Chromatin immunoprecipitations were performed using anti V5-antibody conjugated agarose beads (Sigma-Aldrich) and anti HA antibody conjugated agarose beads (Sigma-Aldrich) as controls

2015-10-29 | E-GEOD-62811 | biostudies-arrayexpress

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

Project description:To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

2023-01-24 | PXD031151 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

2023-01-24 | PXD031154 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET2

2023-01-24 | PXD031152 | Pride

Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET2

2023-01-24 | PXD031153 | Pride

Protein interactions with HA-tagged WDR60 in CEP170 knockout cells

Project description:To identify those proteins interacting with the WDR60 subunit of cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunit WDR60 (DYNC2I1).

2024-04-15 | PXD046844 | Pride

H3K36me3 Reader NPAC/GLYR1 Recruits H3K4 Demethylase LSD2/KDM1B to Modulate Active Gene Transcription

Project description:NPAC ChIP were performed by anti-Flag and anti-HA tandem affinity purification from HeLa stably expressing Flag-HA tagged NPAC from pOZ-N vector, and enrichement on chromosome 3, 21, and 22 were determined by chip microarray analysis using Affymatrix HumanTiling 2.0 arrays

2021-10-31 | GSE160129 | GEO

The interactome of G3BP1 in UMUC3 cells

Project description:The HA-tagged G3BP1 complex was obtained by co-IP with anti-HA antibodies from UMUC3 cells stably expressing HA-tagged G3BP1. The purified immunoprecipitants were resolved on gradient SDS-PAGE, silver-stained, and subjected to MS analysis.

2024-05-23 | PXD035722 | Pride

Aurora-A interactome in MV4-11 cells

Project description:To determine interactors of Aurora-A, HA tagged Aurora-A was immunoprecipitated from MV4-11 cells stably expressing HA tagged Aurora-A wild type and compared to MV4-11 cells expressing empty vector.

2020-09-28 | PXD019684 | Pride

Interactome of chromatin-associated RNA Polymerase II from U2OS cells

Project description:To determine the interactome of RNA Polymerase II (RNAPII), HA-tagged RPB3 was stably expressed in U2OS cells. The cells were harvested followed by the native stepwise isolation of chromatin-associated RNAPII complexes. HA-RPB3 was immunoprecipitated and interacting proteins analyzed by quantitative mass spectrometry. U2OS cells expressing no HA-tagged protein were used for comparison.

2024-12-20 | PXD054701 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data