Dataset Information

Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment

ABSTRACT: Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proof of principle, this approach reveals that in mice exposed to an enriched environment, reduced endocannabinoid synthesis and signaling is linked to increased surface expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) in a subset of Cannabinoid-receptor 1 positive synapses. This mechanism regulates synaptic strength in an input-specific manner. Thus, we establish a compartment-specific multiomics workflow that is suitable to extract information from complex lipid and protein networks involved in synaptic function and plasticity.

INSTRUMENT(S): Direct infusion MS - positive, Direct infusion MS - negative

SUBMITTER: Canan Has Nils Hoffmann

PROVIDER: MTBLS1531 | MetaboLights | 2021-09-27

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS1531	Other
	FILES	Other
	a_MTBLS1531_DI-MS_negative__metabolite_profiling-1.txt	Txt
	a_MTBLS1531_DI-MS_negative__metabolite_profiling.txt	Txt
	a_MTBLS1531_DI-MS_positive__metabolite_profiling-1.txt	Txt

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Publications

Multiomics of synaptic junctions reveals altered lipid metabolism and signaling following environmental enrichment.

Borgmeyer Maximilian M Coman Cristina C Has Canan C Schött Hans-Frieder HF Li Tingting T Westhoff Philipp P Cheung Yam F H YFH Hoffmann Nils N Yuanxiang PingAn P Behnisch Thomas T Gomes Guilherme M GM Dumenieu Mael M Schweizer Michaela M Chocholoušková Michaela M Holčapek Michal M Mikhaylova Marina M Kreutz Michael R MR Ahrends Robert R

Cell reports 20211001 1

Membrane lipids and their metabolism have key functions in neurotransmission. Here we provide a quantitative lipid inventory of mouse and rat synaptic junctions. To this end, we developed a multiomics extraction and analysis workflow to probe the interplay of proteins and lipids in synaptic signal transduction from the same sample. Based on this workflow, we generate hypotheses about novel mechanisms underlying complex changes in synaptic connectivity elicited by environmental stimuli. As a proo ...[more]

PMID: 34610315

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