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Omics score: 1027

Looking for changes in lipid levels with knock out of ACADM (shACADM)

ABSTRACT: GSC 8-11 cells (20 million) were transduced with lentivirus coding for shACADM 4, 6, or scr (control). ACADM is an enzyme responsible for the metabolism of medium chain fatty acids (C4-C12). A round of selection was performed using puromycin to ensure only cells that had been transduced survived. Knockdown of ACADM was confirmed via western blot. Pellets were collected by centrifuging cells for 10 min at max speed. The pellets were washed and each sample was split into quadruplicate.

INSTRUMENT(S): Triple quadrupole

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Cultured Cells

SUBMITTER: Maureen Kachman

PROVIDER: ST000771 | MetabolomicsWorkbench | Mon Jun 19 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

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