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Sub-nanoliter metabolomics via mass spectrometry to characterize volume-limited samples - EBC

ABSTRACT: The human metabolome provides a window into the mechanisms and biomarkers of various diseases. However, because of limited availability, many sample types are still difficult to study by metabolomic analyses. Here, we present a new mass spectrometry (MS)-based metabolomics strategy that only consumes sub-nanoliter sample volumes. The approach consists of combining a customized metabolomics workflow with a pulsed MS ion generation method, known as triboelectric nanogenerator inductive nanoelectrospray ionization (TENGi nanoESI) MS. The first set of samples tested for this approach included exhaled breath condensates (EBC) collected from cystic fibrosis (CF) patients with impaired glucose tolerance to study the metabolome changes before and after the oral glucose tolerance test.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Exhaled Breath Condensate

DISEASE(S): Cystic Fibrosis

SUBMITTER: Fernandez Facundo

PROVIDER: ST001437 | MetabolomicsWorkbench | Wed Jul 29 00:00:00 BST 2020

REPOSITORIES: MetabolomicsWorkbench

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