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Metabolomic and Cultivation Insights into the Tolerance of the Spacecraft-Associated Acinetobacter Towards Kleenol 30, a Cleanroom Floor Detergent

ABSTRACT: To ensure cleanliness, NASA spacecraft are assembled in cleanroom facilities, where floors are routinely cleansed with Kleenol 30 (K30), an alkaline detergent. Through metabolomic and cultivation approaches, we show that cultures of spacecraft-associated Acinetobacter tolerate up to 1% v/v K30 and are fully inhibited at ≥2%; in comparison, NASA cleanrooms are cleansed with 0.8% K30. For A. johnsonii 2P08AA (isolated from a cleanroom floor), cultivations with 0.1% v/v K30 yield (1) limited changes in the intracellular metabolome and (2) increases in extracellular sugar acids, monosaccharides, organic acids, and fatty acids. For A. radioresistens 50v1 (isolated from a spacecraft surface), cultivations yield (1) differential changes in intracellular amino acids, compatible solutes, nucleotide-related metabolites, dicarboxylic acids, and saturated fatty acids and (2) substantial yet differential impacts to extracellular sugar acids, monosaccharides, and organic acids. These combined results suggest that (1) K30 manifests strain-dependent impacts on the intracellular metabolomes and (2) K30 influences extracellular trace element acquisition in both strains. Hence, this work lends support towards the hypothesis that repeated cleansing during spacecraft assembly serve as selective pressures that promote tolerances towards the cleaning conditions.

ORGANISM(S): Acinetobacter

TISSUE(S): Bacterial Cells

SUBMITTER: rakesh mogul

PROVIDER: ST002380 | MetabolomicsWorkbench | Fri Nov 04 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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Metabolomic and Cultivation Insights into the Tolerance of the Spacecraft-Associated Acinetobacter Towards Kleenol 30, a Cleanroom Floor Detergent

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