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Trichoderma reesei RUT C-30 isolate:ATCC 56765

ABSTRACT: Trichoderma reesei RUT C-30 genome sequencing

PROVIDER: PRJNA337417 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR4024940_1.fastq.gz	Fastqsanger.gz
	SRR4024940_2.fastq.gz	Fastqsanger.gz
	SRR4024950_1.fastq.gz	Fastqsanger.gz
	SRR4024950_2.fastq.gz	Fastqsanger.gz
	SRR4024960.fastq.gz	Fastqsanger.gz

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Trichoderma RUT C-30 secretome after growth on different cellulosic substrates

Project description:Enzyme production by T. reesei Rut C-30 was studied in submerged cultures on five different cellulose-rich substrates, namely, commercial cellulose Avicel® and industrial-like cellulosic pulp substrates which consist mainly of cellulose, but also contain residual hemicellulose and lignin. In order to evaluate the hydrolysis of the substrates by the fungal enzymes, the spatial polymer distributions were characterised by cross-polarization magic angle spinning carbon-13 nuclear magnetic resonance (CP/MAS 13C-NMR) in combination with spectral fitting. Proteins in culture supernatants at early and late stages of enzyme production were labeled by Tandem Mass Tags (TMT) and protein profiles were analysed by liquid chromatography-tandem mass spectrometry.

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ArrayCGH of Trichoderma reesei strains QM9123, QM9414, NG14 and RutC-30

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ArrayCGH of Trichoderma reesei strains QM9123, QM9414, NG14 and RutC-30

Project description:Trichoderma reesei is the main industrial producer of cellulases and hemicellulases used to depolymerize biomass in many biotechnical applications. Many production strains in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in hyperproducing mutants of T. reesei by high-resolution comparative genomic hybridisation tiling array. We carried out aCGH analysis of four hyperproducing strains (QM9123, QM9414, NG14 and RutC-30) using QM6a genome as a reference. ArrayCGH analysis identified dozens of mutations in each strain analyzed. 2.1 million oligonucleotide probe custom aCGH (HD2 format, RocheNimblegen) was designed according to T. reesei strain QM6a genome v2.0 (http://genome.jgi-psf.org/Trire2/Trire2.home.html). 14 samples are included in this set; 3 replicates of each strain (except two replicates of QM9123) were analyzed (four mutant strains and QM6a control strain for self-hybridization)

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