ABSTRACT: Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Amanita muscaria MEII Populus tremula x tremuloides metatranscriptome
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Amanita muscaria ectomycorrhizal root tips compared to free-living mycelium . Ectomycorrhizal root tips and control mycelium were harvested after 6 weeks and used for RNA extraction. Reads of 150 bp were generated and aligned to Amanita muscaria transcripts (http://genome.jgi-psf.org/Amamu1) using CLC Genomics Workbench 7. mRNA profiles from Amanita muscaria ectomycorrhizal root tips and free-living mycelium were generated by Illumina HiSeq2000 sequencing (150bp). Two biological replicates were sequenced for mycorrhizal and mycelium samples.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from Amanita muscaria ectomycorrhizal root tips compared to free-living mycelium . Ectomycorrhizal root tips and control mycelium were harvested after 6 weeks and used for RNA extraction. Reads of 150 bp were generated and aligned to Amanita muscaria transcripts (http://genome.jgi-psf.org/Amamu1) using CLC Genomics Workbench 7.
Project description:Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Tulasnella calospora - Serapias vomeracea metatranscriptome
Project description:Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Pisolithus microcarpus 441 Eucalyptus globulus metatranscriptome
Project description:Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Rhyzoscyphus and Meliniomyces - Vaccinium myrtillus metatranscriptome
Project description:Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Oidiodendron maius strain Zn Vaccinium myrtillus metatranscriptome
Project description:Mycorrhizal fungal microbial communities from INRA, France, for Deep Transcriptomics studies - Hebeloma cylindrosporum TV98 IV3 X Pinus pinaster metatranscriptome
Project description:Gene expression changes in Amanita muscaria- Populus tremula x tremuloides ectomycorrhizal root tips compared to Amanita muscaria free-living mycelium
Project description:The Poplar transcriptome was analyzed in mycorrhizal root tips in contact with Laccaria bicolor for 2 weeks. During mycorrhization the roots were treated with either 250M-BM-5m ACC, 10nM JA or 500M-BM-5M SA and compared to untreated mycorrhiza or control roots without contact to L. bicolor. In addition the poplar mutants 35S::PttACO1 and 35S::Atetr1 were used We performed 27 hybridizations (NimbleGen) with samples derived from Populus tremula x Populus alba clone 717-1B4 control roots, untreated mycorrhiza, SA-treated mycorrhiza, ACC-treated mycorrhiza and JA-treated mycorrhiza (3 biological replicates each) as well as Populus tremula x Populus tremuloides T89 control roots, mycorrhiza, 35S::PttACO1 mycorrhiza and 35S::Atetr1-1 mycorrhiza (3 biological replicates). All samples were labeled with Cy3.
