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Solanum lycopersicum cultivar:Moneymaker

ABSTRACT: Changes of tomato transcriptomes upon Tomato yellow leaf curl virus infection by RNA-Seq

PROVIDER: PRJNA480532 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7507770_1.fastq.gz	Fastqsanger.gz
	SRR7507770_2.fastq.gz	Fastqsanger.gz
	SRR7507761_1.fastq.gz	Fastqsanger.gz
	SRR7507761_2.fastq.gz	Fastqsanger.gz
	SRR7507771_1.fastq.gz	Fastqsanger.gz

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Solanum lycopersicum cultivar:Moneymaker

Project description:transcriptome, sRNAome and methylome of tomatoes infected by tomato yellow leaf curl virus (TYLCV)

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Transcriptome profiling of RNAi-trasgenic Solanum lycopersicum plants resistant to Tomato yellow leaf curl virus

Project description:RNA interference (RNAi) is a widely-used approach to generate virus-resistant transgenic crops. However, durability of RNAi-mediated resistance under extreme field conditions and side-effects of stable RNAi expression have not been thoroughly investigated. Here we performed field trials and molecular characterization of two RNAi-transgenic Solanum lycopersicum lines resistant to Tomato yellow leaf curl virus (TYLCV) disease, the major constraint for tomato cultivation in Cuba and worldwide. In order to determine potential impact of the hairpin RNA transgene expression on tomato genome expression and development, differences in the phenotypes and the transcriptome profiles between the transgenic and non-transgenic plants were examined. Transcriptome profiling revealed a common set of up- and down-regulated tomato genes, which correlated with slight developmental abnormalities in both transgenic lines.

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Transcriptional reprogramming in transgenic Arabidopsis plants expressing C4G2A and a cas-1 mutant upon activation of plant immunity.

Project description:Transcriptional overlap between transgenic Arabidopsis plants expressing C4G2A from the Tomato yellow leaf curl virus (TYLCV) and a cas-1 mutant upon activation of plant immunity by treatment with the bacterial peptide elicitor flg22 (1 µM, 12 h).

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Transcriptomic analysis of tomato plants infected by the geminivirus TYLCSV

Project description:Gene expression of tomato plants infected by Tomato yellow leaf curl Sardinia virus (TYLCSV) and mock inoculated were compared.

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Identification of tomato proteins that interact with Replication initiator protein (Rep) of the geminivirus TYLCV

Project description:In this study, an GFP-based affinity purification followed by mass spectrometry (AP-MS) approach is used in order to identify new tomato proteins that interact with the Replication initiator protein (Rep, AL1, C1) from Tomato yellow leaf curl virus (TYLCV).

2020-07-10 | PXD018011 | Pride

Solanum lycopersicum

Project description:Transcriptome profiling of RNAi-trasgenic Solanum lycopersicum plants resistant to Tomato yellow leaf curl virus

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Global Analysis of the Transcriptional Response of Whitefly to Tomato Yellow Leaf Curl China Virus (TYLCCNV)

Project description:We investigated the transcriptional response of invasive B. tabaci B biotype to tomato yellow leaf curl China virus (TYLCCNV) using Illumina sequencing technology. We found that 1,606 genes involved in 157 biochemical pathways were differentially expressed in the viruliferous whiteflies.

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A Crop Plant Integrates and Coordinates its Defenses to Cope with the Attack by Begomovirus and Whitefly

Project description:We analyzed the global defense responses in common tobacco against a begomovirus Tomato yellow leaf curl China virus, an invasive whitefly species Middle East-Asia Minor 1, and their co-infestation. The transcripts of defense related genes were both overlapping and divergent in response to virus and whitefly.

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Geminiviruses employ host DNA glycosylases to subvert DNA methylation-mediated defense

Project description:DNA methylation is an epigenetic mechanism that plays important roles in gene regulation and transposon silencing. Active DNA demethylation has evolved to counterbalance DNA methylation at many endogenous loci. Here, we report that active DNA demethylation also targets viral DNAs, tomato yellow leaf curl China virus (TYLCCNV) and its satellite tomato yellow leaf curl China betasatellite (TYLCCNB), to promote their virulence. We demonstrate that the βC1 protein, encoded by TYLCCNB, interacts with a ROS1-like DNA glycosylase in Nicotiana benthamiana and with the DEMETER (DME) DNA glycosylase in Arabidopsis thaliana. The interaction between βC1 and DME facilitates the DNA glycosylase activity to decrease viral DNA methylation and promote viral virulence. These findings reveal that active DNA demethylation can be regulated by a viral protein to subvert DNA methylation-mediated defense.

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Project description:Characterization and function of Tomato yellow leaf curl virus-derived small RNAs generated in the tolerant and susceptible tomato varieties using deep sequencing

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