Project description:To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(ΔsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains.
Project description:To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(M-NM-^TsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains. 2 ml samples were separately harvested from B. thuringiensis HD73 and HD(M-NM-^TsigL::kan) strains grown in SchaefferM-bM-^@M-^Ys sporulation medium (SSM) at stages T7 of stationary phase (7 hours after the end of the exponential phase). Three independent repeats were performed for each stain.
Project description:We investigated the gene expression and metabolic regulatory mechanisms associated with the high-level accumulation of ICPs by performing the transcriptomics analysis of B. thuringiensis strain CT-43, using Illumina high throughout sequencing (RNA-seq) technique.
Project description:Transcriptional profiling of C. elegans nasp-1 / btr-1 mutant worms versus wild type N2 strain, both exposed to the bacterial pathogen Bacillus thuringiensis DB27.
Project description:Transcriptional profiling of C. elegans nasp-1 / btr-1 mutant worms versus wild type N2 strain, both exposed to the bacterial pathogen Bacillus thuringiensis DB27. One-condition experiment. C. elegans nasp-1 / btr-1 mutant versus N2, exposed to Bacillus thuringiensis DB27. 3 biological replicates, including 1 dye-swaps.
Project description:We investigated the gene expression and metabolic regulatory mechanisms associated with the high-level accumulation of ICPs by performing the transcriptomics analysis of B. thuringiensis strain CT-43, using Illumina high throughout sequencing (RNA-seq) technique. The bacterial cells were collected at the time points of 7 h, 9 h, 13 h and 22 h for the whole-genome transcriptomics, respectively.
Project description:Comparison in late exponential phase (culture OD600 = 3) of global expression profiles from a Bacillus thuringiensis 407 strain overexpressing transcriptional regulator MogR from xylose inducible vector pHT304-Pxyl, versus an isogenic empty vector control strain, to analyze global expression changes resulting from MogR overexpression
Project description:The transcriptional global profiles of the wild-type strain Bacillus thuringiensis 407 and a cdgF deletion mutant were compared after 1.5 hours of growth in an planktonic culture to analyze the effect of deletion of cdgF, which is involved in c-di-GMP signaling, on the global expression profile of B. thuringiensis 407
Project description:Comparison of global expression profiles from a Bacillus thuringiensis 407 strain overexpressing the diguanylate cyclase CdgF (BTB_c06420) from the xylose inducible vector pHT304-Pxyl, versus an isogenic empty vector control strain, to analyze global expression changes resulting from CdgF overexpression
Project description:Transcriptional profiling of C. elegans young adult worms cultured on non-pathogenic Bacillus subtilis strain 67 versus age-matched worms cultured on the control lab food E. coli OP50. The goal was to identify genes regulated in response to differences in diet, which potentially confer immunity to later exposures to pathogenic Bacillus thuringiensis DB27.