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Bulk RNA sequencing of acetylcholine-synthesizing macrophages from subcutaneous adipose tissue

ABSTRACT: Bulk RNA sequencing of acetylcholine-synthesizing macrophages from subcutaneous adipose tissue

PROVIDER: PRJNA729493 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14512105_1.fastq.gz	Fastqsanger.gz
	SRR14512105_2.fastq.gz	Fastqsanger.gz
	SRR14512106_1.fastq.gz	Fastqsanger.gz
	SRR14512106_2.fastq.gz	Fastqsanger.gz
	SRR14512107_1.fastq.gz	Fastqsanger.gz

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Bulk RNA sequencing of acetylcholine-synthesizing macrophages from subcutaneous adipose tissue

Project description:We aimed to study the transcriptomic profile of acetylcholine-synthesizing adipose macrophages. Using a ChAT-eGFP reporter mouse (ChAT, or choline acetyltransferase, is the main enzyme for acetylcholine biosynthesis) acutely exposed to cold temperature, we used FACS to isolate ChAT-eGFP+ and ChAT-eGFP- macrophages from inguinal subcutaneous white adipose tissue (IWAT). We subjected these cells to bulk RNA-seq to better understand the transcriptomic landscape of cholinergic macrophages under cold stress and their role in beige fat thermogenesis.

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