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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila

ABSTRACT: Essential role of Cp190 in physical and regulatory boundary formation in Drosophila

PROVIDER: PRJNA748025 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15192700_1.fastq.gz	Fastqsanger.gz
	SRR15192700_2.fastq.gz	Fastqsanger.gz
	SRR15192701_1.fastq.gz	Fastqsanger.gz
	SRR15192701_2.fastq.gz	Fastqsanger.gz
	SRR15192702_1.fastq.gz	Fastqsanger.gz

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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila

Project description:Animal genomes fold into contact domains defined by enhanced internal contact frequencies with debated functions in establishing independent gene regulatory domains. A large fraction of contact domains in mammals are formed by stalling of chromosomal loop-extruding cohesin by CTCF at domain boundaries. 90% of domain boundaries in Drosophila form CTCF-independently, and other proteins were proposed to form chromosomal loops with dual functions of segregating promoters from inappropriate regulatory elements and connecting distal regulatory elements to their correct targets. Here, we genetically ablate the ubiquitous boundary-associated factor Cp190 and assess impacts on genome folding and transcriptional regulation in embryos. Our results reveal that Cp190 is a major factor required for contact domain boundary formation and gene insulation in Drosophila.

2022-05-30 | PXD027795 | Pride

Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [HiC]

Project description:Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [HiC]

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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [Capture-C]

Project description:Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [Capture-C]

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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [ChIP-seq]

Project description:Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [ChIP-seq]

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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [HiC]

Project description:While Drosophila has widespread contact domains, most domain boundaries are formed independently of CTCF by unclear mechanisms. We genetically ablate the ubiquitous boundary-associated factor Cp190 and examine impacts on genome folding and developmental gene regulation in Drosophila embryos. We find that Cp190 is widely required to form fly domain boundaries that are distal from transcribed gene promoters, while promoter proximal boundaries are unaffected by Cp190 loss.

2022-05-12 | GSE180375 | GEO

Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [ChIP-seq]

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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila [Capture-C]

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Essential role of Cp190 in physical and regulatory boundary formation in Drosophila

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-12 | GSE180376 | GEO

CP190 and CTCF mediated enhancer blocking is augmented by SUMOylation

Project description:Chromatin insulators shield promoters and chromatin domains from neighbouring enhancers or chromatin regions with opposing activities. Insulator binding proteins and their cofactors mediate the boundary function. In general, covalent modification of proteins by the Small Ubiqutin-like Modifier (SUMO) is an important mechanism to control the interaction of proteins within complexes. Here we addressed the impact of SUMO in respect to insulator function, chromatin binding of insulator factors and formation of insulator speckles. SUMOylation augments the enhancer blocking function of four different insulator sequences and increases the genome-wide binding of the insulator cofactor CP190. A model is discussed with enhanced chromatin binding of SUMOylated CP190 causing fusion of insulator speckles, which may allow for more efficient insulation.

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M1BP cooperates with CP190 to activate transcription at TAD borders and promote chromatin insulator activity

Project description:Genome organization is driven by forces affecting transcriptional state, but the relationship between transcription and genome architecture remains unclear. Here, we identified the Drosophila transcription factor Motif 1 Binding Protein (M1BP) in physical association with the gypsy chromatin insulator core complex, including the universal insulator protein CP190. M1BP is required for enhancer-blocking and barrier activities of the gypsy insulator as well as its proper nuclear localization. Genome-wide, M1BP specifically colocalizes with CP190 at Motif 1-containing promoters, which are enriched at topologically associating domain (TAD) borders. M1BP facilitates CP190 chromatin binding at many shared sites and vice versa. Both factors promote Motif 1-dependent gene expression and transcription near TAD borders genome-wide. Finally, loss of M1BP reduces chromatin accessibility and increases both inter- and intra-TAD local genome compaction. Our results reveal physical and functional interaction between CP190 and M1BP to activate transcription at TAD borders and mediate chromatin insulator-dependent genome organization.

2021-06-07 | PXD026497 | Pride

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