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Triton X-100 reference proteome of brain capillary endothelial cells

ABSTRACT: Bovine brain capillary endothelial cells (BBCEC) are cocultured in presence of rat astrocytes to reinduce blood brain barrier properties. After checking of BBB propereties reinduction, BBCEC are harvested by collagenase treatment, lysed with Triton X-100 based lysis buffer. Triton X-100 soluble proteins are subjected to organic fractionation step and the 5 generated fractions were submitted to 1D-LC-MS/MS analysisi on MALDI-TOF-TOF-MS.

INSTRUMENT(S): Bruker Daltonics flex series, instrument model

ORGANISM(S): Bos Taurus (bovine)

TISSUE(S): Cerebrovascular Endothelial Cell, Endothelial Cell, Blood Vessel Endothelial Cell

SUBMITTER: Christophe FLAHAUT

PROVIDER: PRD000250 | Pride | 2010-12-03

REPOSITORIES: Pride

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			Action	DRS
	PRIDE_Exp_Complete_Ac_12825.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_12825.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_12825.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_12826.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_12826.pride.mztab.gz	Mztab

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