Dataset Information

Interspecies comparison of GNA binding proteins

ABSTRACT: A very common co-translational modification in multicellular organisms is the addition of carbohydrate structures called glycans to the peptide backbone. This important protein modification is called protein glycosylation. In this report, the diversity in protein glycosylation between insect species covering a wide phylogenetic distance was analyzed. Based on previous data of the glycan profile from the fruit fly (Drosophila melanogaster), the oligomannose N- glycan binding snowdrop lectin (Galanthus nivalis agglutinin, GNA) was selected for affinity chromatography of glycoproteins. The insect species that were used in this study were the flour beetle (Tribolium castaneum), the silkworm (Bombyx mori), the honey bee (Apis mellifera), the fruit fly (D. melanogaster) and the pea aphid (Acyrthosiphon pisum). To identify and characterize the purified glycoproteins, LC-MS/MS analysis was performed. For all insect species, it was demonstrated that glycoproteins are involved in a broad range of biological processes and molecular functions. However, major differences between insect species were observed for particular glycosylated proteins. This finding was reflected by differences in the relative abundance of particular proteins for certain biological processes or molecular functions. Besides the abundant presence of oligomannose N-glycans on insect glycoproteins, we also showed that O-mannosylation events occur more frequently than currently anticipated.

INSTRUMENT(S): instrument model, Bruker Daltonics amaZon series

ORGANISM(S): Acyrthosiphon Pisum (pea Aphid) Bombyx Mori (silk Moth) Tribolium Castaneum (red Flour Beetle) Drosophila Melanogaster (fruit Fly) Apis Mellifera (honeybee)

SUBMITTER: Bart Ghesquiere

PROVIDER: PRD000276 | Pride | 2011-03-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_13290.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_13290.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_13290.xml.gz	Xml

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Publications

Diversity in protein glycosylation among insect species.

Vandenborre Gianni G Smagghe Guy G Ghesquière Bart B Menschaert Gerben G Nagender Rao Rameshwaram R Gevaert Kris K Van Damme Els J M EJ

PloS one 20110223 2

<h4>Background</h4>A very common protein modification in multicellular organisms is protein glycosylation or the addition of carbohydrate structures to the peptide backbone. Although the Class of the Insecta is the largest animal taxon on Earth, almost all information concerning glycosylation in insects is derived from studies with only one species, namely the fruit fly Drosophila melanogaster.<h4>Methodology/principal findings</h4>In this report, the differences in glycoproteomes between insect ...[more]

PMID: 21373189

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