Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Glycosylation signatures in Drosophila: fishing with lectins

ABSTRACT: enrichment of glycoproteins in fruit fly (Drosophila) using GNA, RSA and Nictaba lectin

INSTRUMENT(S): Bruker Daltonics HCT Series, instrument model

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Bart Ghesquiere

PROVIDER: PRD000822 | Pride | 2011-03-18

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_12011.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_12011.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_12011.xml.gz	Xml

Items per page:

1 - 3 of 3

Publications

Glycosylation signatures in Drosophila: fishing with lectins.

Vandenborre Gianni G Van Damme Els J M EJ Ghesquière Bart B Menschaert Gerben G Hamshou Mohamad M Rao Rameshwaram Nagender RN Gevaert Kris K Smagghe Guy G

Journal of proteome research 20100601 6

Glycosylation is a co- and/or post-translational protein modification that generates enormous structural diversity among glycoproteins. In this study, immobilized lectins were used to capture glycoproteins with different glycan profiles from Drosophila melanogaster extracts. On the basis of previous results from glycan array analyses, the snowdrop (Galanthus nivalis) agglutinin (GNA), the tobacco (Nicotiana tabacum) lectin (Nictaba) and the Rhizoctoni solani agglutinin (RSA) were used to select ...[more]

PMID: 20387871

Similar Datasets

interspecies comparison of GNA binding proteins

Project description:A very common co-translational modification in multicellular organisms is the addition of carbohydrate structures called glycans to the peptide backbone. This important protein modification is called protein glycosylation. In this report, the diversity in protein glycosylation between insect species covering a wide phylogenetic distance was analyzed. Based on previous data of the glycan profile from the fruit fly (Drosophila melanogaster), the oligomannose N- glycan binding snowdrop lectin (Galanthus nivalis agglutinin, GNA) was selected for affinity chromatography of glycoproteins. The insect species that were used in this study were the flour beetle (Tribolium castaneum), the silkworm (Bombyx mori), the honey bee (Apis mellifera), the fruit fly (D. melanogaster) and the pea aphid (Acyrthosiphon pisum). To identify and characterize the purified glycoproteins, LC-MS/MS analysis was performed. For all insect species, it was demonstrated that glycoproteins are involved in a broad range of biological processes and molecular functions. However, major differences between insect species were observed for particular glycosylated proteins. This finding was reflected by differences in the relative abundance of particular proteins for certain biological processes or molecular functions. Besides the abundant presence of oligomannose N-glycans on insect glycoproteins, we also showed that O-mannosylation events occur more frequently than currently anticipated.

2011-03-18 | PRD000276 | Pride

Identification of Cbt target genes in Drosophila wing imaginal discs

Project description:We performed a genome-wide ChIP analysis to find the set of target genes regulated by Cbt in Drosophila wing imaginal discs. We reported multiple transcriptional regulators and genes involved in the developlment, growth and patterning of the fruit fly.

2015-02-20 | GSE40958 | GEO

Shared Transcriptomic Signatures of Inflammaging Among Diverse Strains of Drosophila melanogaster

Project description:Background: A prominent hallmark of aging is inflammaging—the increased expression of innate immune genes without identifiable infection. Model organisms with shorter lifespans, such as the fruit fly, provide an essential platform for probing the mechanisms of inflammaging. Multiple groups have reported that, like mammalian models, old flies have significantly higher levels of expression of anti-microbial peptide genes. However, whether some of these genes—or any others—can serve as reliable markers for assessing and comparing inflammaging in different strains remains unclear. Methods and Results: We compared RNA-Seq datasets generated by different groups. Although the fly strains used in these studies differ significantly, we found that they share a core group of genes with strong aging-associated expression. In addition to anti-microbial peptide genes, we identified other genes that have prominently increased expression in old flies, especially SPH93. We further showed that machine learning models can be used to predict the “inflammatory age” of the fruit fly.

2024-04-17 | GSE262759 | GEO

Traumatic brain injury in Drosophila causes retention of long introns.

Project description:Traumatic brain injury (TBI) can cause persistent pathological alteration of neurons. This may lead to cognitive dysfunctions, depression, and even increased susceptibility to life threatening diseases, such as epilepsy. To investigate the underlying genetic and molecular basis of TBI, Wasserman and colleagues developed an inexpensive and reproducible model for simulating TBI in Drosophila melanogaster (Fruit Fly). Using a modified version of this model, we subjected w1118 fruit flies to mild closed head trauma. To determine the transcriptomic changes which contribute to survival post TBI, we collected fly heads from the survivors at 2 time points; 4 hours and 24 hours post-trauma. We observed that the induction of transcriptomic changes occur 24 hours post-trauma in fly heads. Classification of these AS changes showed selective retention of long introns (>81bps), with a mean size of ~3000bps. Gene ontology analysis of the genes containing retained introns showed significant enrichment for critical regulators of glycolysis, citric acid cycle as well as vesicle mediated neurotransmitter release. Some of these genes also showed a significant reduction in transcript abundance. The retained introns were enriched for CA-rich motifs known to bind to Smooth (SM), an hnRNPL class of splicing factor.

2017-09-22 | GSE104100 | GEO

The small RNA profile in wildtype, R2D2 and loquacious mutant Drosophila

Project description:We used SOLiD technology to deep sequence libraries prepared from 18-35 nt PAGE purfied small RNAs derived from female Drosophila. The objective of our study was to compare the impact of R2D2 or loquacious mutantions on different small RNA populations in the fruit fly.

2009-11-04 | GSE18871 | GEO

Expression data for dSTING mutant vs. wild type flies

Project description:STING is a protein that plays important role in innate immune response. However, it also has functions not related to immunity. We studied role of STING in fruit fly Drosophila melanogaster. We used microarray to detect gene expression changes in dSTING knockout fruit flies. We studied role of STING in fruit fly Drosophila melanogaster. To detect gene expression changes in dSTING-knockout flies microarray assay was used.

2021-02-22 | GSE167164 | GEO

mini-INTACT reveals social isolation-induced epigenetic changes in Drosophila dopaminergic neurons

Project description:We introduce a more sensitive method for purifying specific cell-types from small numbers of fly heads for epigenomic analysis: a method we call ‘mini-INTACT’. We modified the INTACT method in Drosophila [Henry G. L. et al., Nucleic Acids Research, 2012, 1–14] to enable purification of genetically tagged rare cell types from Drosophila brain requiring ~50-100 fold less material. We used male fruit flies as a model to examine the effects of social-isolation vs. social-enrichment on the epigenome and transcriptome of dopaminergic neurons defined by expression of the TH-GAL4 driver. Using ChIP-seq on mini-INTACT purified nuclei, RNA-seq and bioinformatic analysis, we identify regulation of the transcriptome and behavior by Activity Related Genes who’s expression is induced by social enrichment.

2019-02-07 | GSE126186 | GEO

Effects of upregulated JAK/STAT signaling on gene expression in Drosophila airway epithelial cells

Project description:Chronically activated JAK/STAT signaling leads to several lung diseases, including lung cancer, asthma, and chronic obstructive pulmonary disease. We used the fruit fly Drosophila melanogaster to elucidate the molecular processes which transform elevated JAK/STAT signaling in airway epithelial cells into pathology.

2022-08-31 | GSE212132 | GEO

The small RNA profile in wildtype, R2D2 and loquacious mutant Drosophila

2009-11-04 | E-GEOD-18871 | biostudies-arrayexpress

Genomics analysis of L-DOPA exposure in Drosophila sechellia

Project description:The dietary specialist fruit fly Drosophila sechellia evolved to feed primarily on the toxic fruit of Morinda citrifolia. Seeking the basis of adaptations to the unique chemistry of its host plant, we profiled genome-wide gene expression response to L-DOPA because it is highly abundant in M. citrifolia, critical for reproductive success of D. sechellia, and may influence toxin resistance in this species. Here we used a combination of functional genetics and genomics to identify a new gene, Esterase 6 (Est6) that is important for D. sechellia ecological adaptation to this new niche.

2019-09-28 | GSE138119 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data