Dataset Information

Head-to-head comparison of serum fractionation techniques

ABSTRACT: Not available

INSTRUMENT(S): instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Attila Csordas

PROVIDER: PRD000463 | Pride | 2012-07-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_17907.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17907.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_17907.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_17908.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_17908.pride.mztab.gz	Mztab

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Publications

Head-to-head comparison of serum fractionation techniques.

Whiteaker Jeffrey R JR Zhang Heidi H Eng Jimmy K JK Fang Ruihua R Piening Brian D BD Feng Li-Chia LC Lorentzen Travis D TD Schoenherr Regine M RM Keane John F JF Holzman Ted T Fitzgibbon Matthew M Lin ChenWei C Zhang Hui H Cooke Kelly K Liu Tao T Camp David G DG Anderson Leigh L Watts Julian J Smith Richard D RD McIntosh Martin W MW Paulovich Amanda G AG

Journal of proteome research 20070201 2

Multiple approaches for simplifying the serum proteome have been described. These techniques are generally developed across different laboratories, samples, mass spectrometry platforms, and analysis tools. Hence, comparing the available schemes is impossible from the existing literature because of confounding variables. We describe a head-to-head comparison of several serum fractionation schemes, including N-linked glycopeptide enrichment, cysteinyl-peptide enrichment, magnetic bead separation ( ...[more]

PMID: 17269739

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Project description:BackgroundFor optimal fertility testing, serum anti-Müllerian hormone levels are used in combination with other testing to provide reliable ovarian reserve evaluations. The use of the ADx 100 card is widely commercially available for at-home reproductive hormone testing, but data demonstrating that its results are reproducible outside of a clinical setting are limited, as well as comparisons of its performance with other newer blood collection techniques. This study aimed to evaluate the concordance of serum AMH levels found via standard venipuncture and self-administered blood collection using the TAP II device (TAP) and ADx card in women of reproductive age.MethodsThis was a prospective, head-to-head-to-head within-person crossover comparison trial that included 41 women of reproductive age (20-39 years). It was hypothesized that the TAP device would be superior to the ADx card both in terms of agreement with venipuncture reference standard and patient experience. Each subject had their blood drawn using the three modalities (TAP, ADx, and venipuncture). We evaluated the concordance of AMH assays from samples obtained via the TAP device and ADx card with the gold standard being venipuncture. Two-sided 95% CIs were generated for each method to compare relative performance across all three modes. Patient preference for the TAP device versus the ADx card was based on self-reported pain and Net Promoter Score (NPS).ResultsThe TAP device was superior to the ADx card on all outcome measures. TAP R-squared with venipuncture was 0.99 (95% CI 0.99, > 0.99), significantly higher than the ADx card, which had an R-squared of 0.87 (95% CI 0.80, 0.94) under most favorable treatment. TAP sensitivity and specificity were both 100% (no clinical disagreement with venipuncture), versus 100 and 88%, respectively, for the ADx card. Average pain reported by users of the TAP device was significantly lower than the ADx card (0.75 versus 2.73, p < 0.01) and the NPS was significantly higher than the ADx card (+ 72 versus - 48, p < 0.01).ConclusionsThe TAP was non-inferior to venipuncture and superior to the ADx card with respect to correlation and false positives. Moreover, the TAP was superior to both alternatives on patient experience.Trial registrationNCT04784325 (Mar 5, 2021).