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Shortgun Proteomic analysis of two Bartonella quintana strains

ABSTRACT: Proteomic analysis two strains of Bartonella quintana using In-solution digestion followed by Stron Cation Exchange (SCX) and RP nano-LC-MS/MS

INSTRUMENT(S): QSTAR XL

ORGANISM(S): Bartonella Quintana Jk 31 Bartonella Quintana (rochalimaea Quintana)

SUBMITTER: Shibu Krishnan

LAB HEAD: Shibu Krishnan

PROVIDER: PXD000076 | Pride | 2013-06-03

REPOSITORIES: Pride

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			Action	DRS
	JK31-1-Stage-2D-Gen-10-2012-01.wiff	Wiff
	JK31-1-Stage-2D-Gen-10-2012-02.wiff	Wiff
	JK31-1-Stage-2D-Gen-10-2012-03.wiff	Wiff
	JK31-1-Stage-2D-Gen-10-2012-04.wiff	Wiff
	JK31-1-Stage-2D-Gen-10-2012-05.wiff	Wiff

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Publications

Shotgun proteomic analysis of two Bartonella quintana strains.

Fabietti Alessandra A Gaspari Marco M Krishnan Shibu S Quirino Angela A Liberto Maria Carla MC Cuda Giovanni G Focà Alfredo A

Proteomics 20130315 8

This study reports the first extensive shotgun analysis of the Bartonella quintana proteome. Proteins extracted from two B. quintana strains, Oklahoma and JK31, were analyzed in triplicate analyses by a bottom-up approach consisting of tryptic digestion in SDS-containing buffer, strong cation-exchange StageTip fractionation, and nano-LC-MS/MS analysis. By setting spectral false discovery rate below 0.5%, 548 unique proteins were identified overall, of which 409 protein identifications were share ...[more]

PMID: 23450663

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