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Comparative Proteome Profiling of Bovine and Human Staphylococcus epidermidis Strains to Search for Cell-surface Bound Factors with Plausible Roles in Virulence or Adaptation

ABSTRACT: Here, we analyse two SE strains, an infectious bovine strain PM221 isolated from persistent subclinical bovine IMI, and ATCC12228 representing a low-infectious human skin strain, on total proteome level.

INSTRUMENT(S): QSTAR

ORGANISM(S): Staphylococcus Epidermidis

SUBMITTER: Pia SiljamÃ¤ki

LAB HEAD: Tuula A Nyman

PROVIDER: PXD000404 | Pride | 2014-06-10

REPOSITORIES: Pride

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			Action	DRS
	100223Elfr136ul.wiff	Wiff
	100223Elfr136ul.wiff.scan	Wiff
	100223Elfr236ul.wiff	Wiff
	100223Elfr236ul.wiff.scan	Wiff
	100223Elfr336ul.wiff	Wiff

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Comparative proteome profiling of bovine and human Staphylococcus epidermidis strains for screening specifically expressed virulence and adaptation proteins.

Siljamäki Pia P Varmanen Pekka P Kankainen Matti M Pyörälä Satu S Karonen Taru T Iivanainen Antti A Auvinen Petri P Paulin Lars L Laine Pia K PK Taponen Suvi S Simojoki Heli H Sukura Antti A Nyman Tuula A TA Savijoki Kirsi K

Proteomics 20140710 16

The present study reports a comparative proteome cataloging of a bovine mastitis and a human-associated Staphylococcus epidermidis strain with a specific focus on surfome (cell-wall bound and extracellular) proteins. Protein identification by 1DE coupled with LC-MS/MS analyses resulted in 1400 and 1287 proteins from the bovine (PM221) and human (ATCC12228) strains, respectively, covering over 50% of all predicted and more than 30% of all predicted surfome proteins in both strains. Comparison of ...[more]

PMID: 24909406

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Genomics and Proteomics Provide New Insight into the Commensal and Pathogenic Lifestyles of Bovine and Human-associated Staphylococcus epidermidis

Project description:The goal of the present study is to understand mechanisms by which SE can infect the bovine host and persist in the mammary gland. For this purpose we determined the whole genome and four plasmid sequences of the SE strain PM221 originating from spontaneous persistent bovine IMI. We compared the genomic data with those of ATCC12228 and RP62A to screen for conserved and acquired gene pools. To confirm and complement these findings two-dimensional difference gel electrophoresis (2D DIGE) involving each three strains, surfome shaving and experimental infections in a bovine model was applied to indicate potential differences.

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Comparative Exoprotein Profiling of Different Staphylococcus epidermidis strains Reveals Potential Link between Non-classical Protein Export and Virulence

Project description:This study reports global exoproteome profiling of bovine mastitis strain PM221 and two human strains, commensal-type ATCC12228 and sepsis-associated RP62A to screen for factors underlying adaptation and virulence.

2014-06-04 | PXD000631 | Pride

A proteomic characterization reveals differences in the milk fat globule membrane of buffalo and bovine milk

Project description:The proteins of the milk fat globule membrane (MFGM) have a number of functions, such as the regulation of milk fat secretion and metabolism, the uptake and transportation of fatty acids in the intestine and potential protection from bacterial or viral infection. While the proteome of the MFGM in bovine milk has been extensively characterised, our knowledge of these proteins in buffalo milk is limited. In this study, a proteomic approach was used to characterise the proteome of the buffalo MFGM. Multiple extraction techniques were employed to increase the coverage of proteins identified, while label free relative quantitative liquid chromatography tandem mass spectrometry was used for comparison between the buffalo and bovine MFGM proteome. A total of 220 buffalo MFGM proteins and 234 bovine MFGM proteins were identified after being filtered from the initial dataset of 757 and 680 proteins respectively. Significantly greater amounts of xanthine oxidoreductase, platelet glycoprotein 4, heat shock cognate and calcineurin B homologous protein were identified per mass of buffalo MFGM protein extracted. The higher expression of xanthine oxidase in the MFGM of buffalo milk was confirmed by Western blot analysis and a heterogeneous distribution of this protein observed in situ on the surface of the MFGM. The high concentration of fat in buffalo milk, together with the differences in the MFGM proteome indicate differences in the nutritional profile, biological function, and potential ease of processing of buffalo and bovine milk products.

2017-05-19 | PXD005318 | Pride

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An integrative transcriptomic and proteomic analysis of an industrial clavulanic acid-overproducing Streptomyces clavuligerus

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2022-11-02 | PXD032272 | Pride

Phosphoproteome characterization of Sendai-virus infection in human epithelial cells

Project description:We used phosphoproteomics combined with bioinformatics to characterize the global cellular protein phosphorylation events during Sendai virus infection in human lung epithelial cells.

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Uterine infection-induced transcriptomic changes in the bovine endometrium

Project description:Purpose: Perform RNA-seq study on infectious bovine endometrial tissues to reveal important genes and biological pathways regulating uterine physiology following uterine infections Methods:RNA sequencings were done using Illumina platform. Single-end reads in the FASTQ format were explored using FastQC, low-quality reads were trimmed from both 3’ and 5’ ends until a base pair of Phred quality score of 30 (99.9% accurate) or greater was found, reads having a mean quality score less than 30 and length below 30 nucleotides were filtered out. Cleaned reads were aligned against the bovine reference genome (Bos_taurus.ARS-UCD1.2) using HiSAT2. The resulting SAM files were sorted, converted to BAM files using SAMtools. Read counts mapped to bovine gene models were generated using htseq-count script from HTSeq package. Bioconductor DESeq2 was used to get the differentially expressed genes among infectious vs normal uterine tract groups Conclusions: The study demonstrated that uterine infections altered several genes and pathways related to inflammatory response, immune response, uterine physiology, uterine enviroment and fertility in the intercaruncular region of bovine endometrium.

2022-07-28 | GSE209700 | GEO

Phosphoproteomics combined with quantitative 14-3-3-affinity capture identifies SIRT1 and RAI as new regulators of cytosolic dsRNA-induced innate immune response

Project description:In this study, we have used global phosphoproteomic analysis to define phosphorylation-dependent signaling events regulated during RLR stimulation in human keratinocytes. In addition, we used quantitative 14-3-3-affinity capture to identify major target molecules of 14-3-3 regulation.

2014-07-08 | PXD000670 | Pride

Transcription profiling of bovine peripheral blood mononuclear cells in intramammary infusion of Mycoplasma bovis

Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine peripheral blood mononuclear cells play an important role for mycoplasma mastitis, however, the effects of M. bovis for immune response of peripheral blood mononuclear cells have not been fully clarified.We examined the transcription profiling of bovine peripheral blood mononuclear cells in intramammary infusion of M. bovis at day 7.

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Transcription profiling of bovine neutrophils stimulated with Mycoplasma bovis against untreated controls

Project description:Mycoplasma species are highly contagious pathogens, and Mycoplasma infectious disease are a serious issue for the dairy industry. The bovine neutrophils play an important role for the eradication of pathogens which cause mycoplasmal infection, however the effects of M. bovis for immune response of neutrophils have not been fully clarified. We examined the transcription profiling of bovine neutrophils on the stimulation with M. bovis for 3h (3 stimuli, 3 control).

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