Dataset Information

Identifcation of Adiponectin and Evidence for Post-Translational Modifications in the Bottlenose Dolphin (Tursiops truncatus)

ABSTRACT: A Multiple Affinity Removal System (MARS) Human 14 spin cartridge was used to deplete a dolphin serum which was then digested with trypsin and analyzed without prior fractionation by nanoLC-MS/MS. These files are associated with "2011-9-25_Neely-DolphinSerum." Data analysis: raw data generated by the AB Sciex 5600 were converted to a peak list using the AB Sciex MS Data Converter (v. 1.1 beta, July 2011). Protein identifications were made using Mascot (v. 2.3.02) searching against the Ensembl (release 64) turTru1 dolphin genome assembly protein database [16,598 sequences] and the common Repository of Adventitious Proteins database (cRAP; 2012.01.01; the Global Proteome Machine) using the following parameters: trypsin was selected as the enzyme and three missed cleavages were allowed; carbamidomethylation (Cys) was specified as a fixed modification; glucosylgalactosyl (Lys), oxidation (Met, Pro), and 2-succinyl (Cys) were specified as variable modifications; a precursor tolerance of 10 ppm and fragment ion tolerance of 0.5 Da; instrument type was ESI-QUAD-TOF. Additionally, a non-depleted dolphin sera that had been digested followed by quasi-fractionation using SPE, was analyzed by nano-LC-MS/MS which lead to an additional peptide identification, KGEPGESAYVYR, and these files are associated with "2012-12-12 Dolphin IDA".

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Tursiops Truncatus (atlantic Bottle-nosed Dolphin) (delphinus Truncatus)

SUBMITTER: Benjamin Neely

PROVIDER: PXD000431 | Pride | 2013-09-23

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2011-9-25_Neely-DolphinSerum.mgf	Mgf
	2011-9-25_Neely-DolphinSerum.wiff	Wiff
	2011-9-25_Neely-DolphinSerum.wiff.mtd	Wiff
	2011-9-25_Neely-DolphinSerum.wiff.scan	Wiff
	2012-12-12DolphinIDA.mgf	Mgf

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Publications

Ratiometric Measurements of Adiponectin by Mass Spectrometry in Bottlenose Dolphins (Tursiops truncatus) with Iron Overload Reveal an Association with Insulin Resistance and Glucagon.

Neely Benjamin A BA Carlin Kevin P KP Arthur John M JM McFee Wayne E WE Janech Michael G MG

Frontiers in endocrinology 20130920

High molecular weight (HMW) adiponectin levels are reduced in humans with type 2 diabetes and insulin resistance. Similar to humans with insulin resistance, managed bottlenose dolphins (Tursiops truncatus) diagnosed with hemochromatosis (iron overload) have higher levels of 2 h post-prandial plasma insulin than healthy controls. A parallel reaction monitoring assay for dolphin serum adiponectin was developed based on tryptic peptides identified by mass spectrometry. Using identified post-transla ...[more]

PMID: 24065958

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