Dataset Information

Symbiont proteome data of whiteflies

ABSTRACT: To examined the effect of the antibiotic treatment on protein expression of symbionts in the whitefly. Proteome analysis showed that 23 Hamiltonella proteins were down-regulated in Hamiltonella-cured (–HBt) compared to Hamiltonella-infected (+HBt) whiteflies (Dataset S1A,B), this data including all protein data of Bemisia tabaci and its symbionts. The resulting MS/MS data were processed using Maxquant search engine (v.1.5.2.8). The tandem mass spectra were searched against genome database of Portiera, Hamiltonella and Rickettsia in B. tabaci MEAM1 (http://www.whiteflygenomics.org/cgi-bin/bta/index.cgi) concatenated with reverse decoy database. Trypsin/P was specified as cleavage enzyme allowing up to 2 missing cleavages. The mass tolerance for precursor ions was set as 20 ppm in First search and 5 ppm in Main search, and the mass tolerance for fragment ions was set as 0.02 Da. Carbamidomethyl on Cys was specified as fixed modification and oxidation on Met was specified as variable modifications. FDR was adjusted to < 1% and minimum score for peptides was set > 40. KEGG database was used to annotate protein pathway using KEGG online service tools KAAS. Then each protein was mapped to the KEGG pathway database using KEGG online service tools KEGG mapper.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rickettsia Sp. Meam1 (bemisia Tabaci)

TISSUE(S): Whole Body

SUBMITTER: Yalin Yao

LAB HEAD: Yalin Yao

PROVIDER: PXD038323 | Pride | 2023-02-14

REPOSITORIES: Pride

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Source:

			Action	DRS
	KA359LQB1.zip	Other
	KA359LQB1_CK1_Slot2-7_1_10349.d.zip	Other
	KA359LQB1_CK2_Slot2-7_1_10350.d.zip	Other
	KA359LQB1_CK3_Slot2-7_1_10351.d.zip	Other
	KA359LQB1_FH1_Slot2-8_1_10353.d.zip	Other

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Symbiont proteome data of whiteflies

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