Dataset Information

Analysis of pummelo proteome using iTRAQ

ABSTRACT: For protein identification, the following parameters were used. Peptide mass tolerance = 20 ppm, Missed cleavage = 2, MS/MS tolerance = 0.1 Da, Enzyme = Trypsin, Fixed modification: Carbamidomethyl (C), iTRAQ8plex(K), iTRAQ8plex(N-term), Variable modification：Oxidation(M)，Decoy database pattern=Reverse. The MASCOT search results for each SCX elution were further processed using the ProteomicsTools (version 3.1.6) which includes the programs BuildSummary, Isobaric Labeling Multiple File Distiller and Identified Protein iTRAQ Statistic Builder (Information can be accessed from Research Center for Proteome Analysis (http://www.proteomics.ac.cn/).

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Citrus Maxima (pomelo) (citrus Grandis)

TISSUE(S): Plant Cell, Flower Bud

SUBMITTER: Beibei Zheng

LAB HEAD: Wen-Wu Guo

PROVIDER: PXD000527 | Pride | 2020-01-27

REPOSITORIES: Pride

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Publications

iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo.

Zheng Bei-Bei BB Fang Yan-Ni YN Pan Zhi-Yong ZY Sun Li L Deng Xiu-Xin XX Grosser Jude W JW Guo Wen-Wu WW

Journal of proteome research 20140527 6

Comprehensive and quantitative proteomic information on citrus floral bud is significant for understanding male sterility of the cybrid pummelo (G1+HBP) with nuclear genome of HBP and foreign mitochondrial genome of G1. Scanning electron microscopy and transmission electron microscopy analyses of the anthers showed that the development of pollen wall in G1+HBP was severely defective with a lack of exine and sporopollenin formation. Proteomic analysis was used to identify the differentially expre ...[more]

PMID: 24824475

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