Dataset Information

In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa

ABSTRACT: Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography - electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These proteins are functionally related to sperm motility and energy production as well as the protection of sperm against oxidative injury and stress. Moreover, carp sperm is equipped with functionally diverse proteins involved in signal transduction, transcription, translation, protein turnover and transport. About 15% of carp sperm proteins identified here were also detected in seminal plasma which may be a result of leakage from spermatozoa into seminal plasma, adsorption of seminal plasma proteins on spermatozoa surface, and expression in both spermatozoa and cells secreting seminal plasma proteins. The availability of a catalogue of carp sperm proteins provides substantial advances for an understanding of sperm function and for future development of molecular diagnostic tests of carp sperm quality, the evaluation of which is currently limited to certain parameters such as sperm count, morphology and motility or viability.

INSTRUMENT(S): LTQ

ORGANISM(S): Cyprinus Carpio (common Carp)

TISSUE(S): Spermatozoon, Sperm

SUBMITTER: Mariola Dietrich

LAB HEAD: Mariola A. Dietrich

PROVIDER: PXD000877 | Pride | 2014-10-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	spermfresh1.dat-pride.xml-merged.pride.mgf.gz	Mgf
	spermfresh1.dat-pride.xml-merged.pride.mztab.gz	Mztab
	spermfresh1.dat-pride.xml-merged.xml.gz	Xml
	spermfresh1band01.RAW	Raw
	spermfresh1band01.RAW.mgf	Mgf

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Publications

In-depth proteomic analysis of carp (Cyprinus carpio L) spermatozoa.

Dietrich Mariola A MA Arnold Georg J GJ Fröhlich Thomas T Ciereszko Andrzej A

Comparative biochemistry and physiology. Part D, Genomics & proteomics 20140928

Using a combination of protein fractionation by one-dimensional gel electrophoresis and high performance liquid chromatography-electrospray ionization tandem mass spectrometry, we identified 348 proteins in carp spermatozoa, most of which were for the first time identified in fish. Dynein, tubulin, HSP90, HSP70, HSP60, adenosylhomocysteinase, NKEF-B, brain type creatine kinase, mitochondrial ATP synthase, and valosin containing enzyme represent high abundance proteins in carp spermatozoa. These ...[more]

PMID: 25305539

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