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Integrating transcriptomics and proteomics data for accurate assembly and annotation of genomes

ABSTRACT: Deep proteomic analysis of larvae, pupae and 15 adult tissues using high resolution Fourier transform mass spectrometry provided peptide evidence for ~9,000 computationally predicted genes accounting for 82% of total protein-coding genes in An. stephensi. Transcriptomic profiling of 4 tissues provided transcript evidence for 7,504 computationally predicted gene models and resulted in identification of 3,797 novel transcripts. We also identified protein level evidence for several novel alternative transcripts, SNPs and tissue restricted expression patterns of several proteins in An. stephensi

INSTRUMENT(S): LTQ Orbitrap Velos, LTQ Orbitrap Elite, Q Exactive

ORGANISM(S): Anopheles Stephensi (asian Malaria Mosquito)

TISSUE(S): Other Source, Testis, Brain, Head, Midgut, Malpighian Tubule, Hemolymph, Thorax, Fat Body, Salivary Gland, Leg, Pupa, Ovary, Larva, Accessory Sex Gland, Proboscis, Antenna

SUBMITTER: Akhilesh Pandey

LAB HEAD: Akhilesh Pandey

PROVIDER: PXD001128 | Pride | 2016-09-26

REPOSITORIES: Pride

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			Action	DRS
	An_Steph_Antennae_IG_SDA_500.msf	Msf
	An_Steph_Max_palp_IG_SDA-500.msf	Msf
	An_Steph_Max_palp_IG_SDA-500.pep.xml	Pepxml
	An_Steph_Proboscis_IG_SDA-500.msf	Msf
	An_Steph_Proboscis_IG_SDA-500.pep.pep.xml	Pepxml

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