Dataset Information

Redox proteomics of Arabidopsis cells under salicylate and flg22 treatments

ABSTRACT: The redox-sensitive proteins were identified in Arabidopsis cells under the treatment of salicylate or flg22 (a peptide from bacterial flagellin) using OxiTRAQ method. Proteins were extracted from cell samples in the presence of NEM to block free thiols. Reversibly oxidized thiols were reduced by DTT and then tagged with biotin-HPDP. After trypsin digestion and affinity purification of the biotinylated peptides, the different samples were labeled with different iTRAQ reagent and then pooled together. Peptides were identified and quantified by tandem MS. In parallel, total protein abundance was determined by regular iTRAQ assay.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Cell Suspension Culture

SUBMITTER: Pei Liu

LAB HEAD: Yiji Xia

PROVIDER: PXD001349 | Pride | 2015-03-03

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Oxi1a.dat-pride.pride.mgf.gz	Mgf
	Oxi1a.dat-pride.pride.mztab.gz	Mztab
	Oxi1a.dat-pride.xml.gz	Xml
	Oxi1b.dat-pride.pride.mgf.gz	Mgf
	Oxi1b.dat-pride.pride.mztab.gz	Mztab

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Publications

Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis.

Liu Pei P Zhang Huoming H Yu Boying B Xiong Liming L Xia Yiji Y

Scientific reports 20150227

Accumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed to alter functions of redox-sensitive proteins through their oxidative modifications. Here we report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines underwent oxidative modificatio ...[more]

PMID: 25720653

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