Dataset Information

Proteogenomic analysis of Anopheles gambiae phagocytic hemocyte populations reveals an anticipatory innate immune response in the absence of pathogen challenge

ABSTRACT: The innate immune response to a broad class of pathogens is highly conserved across all eukaryotes and has been studied in great detail at the cellular and transcriptomic level in several insect species. However, the commensurate cellular proteomic response, especially of hemocytes, the primary immune cell population in insects, has remained poorly understood. We report on the comprehensive proteogenomic analysis of a phagocyte subpopulation from Anopheles gambiae, the primary malaria mosquito vector in Sub-Saharan Africa. We leveraged the innate phagocytic response of mosquito granulocytes to achieve targeted enrichment for these cells to facilitate the examination of their proteomic response profiles following sugar feeding, as well as non-infectious and infectious blood feeding with Plasmodium falciparum. A comparative integrative-OMICs analysis of existing transcriptomic profiles combined with these proteomic data permitted the delineation of the functional genome of anopheline granulocytes. We observed that phagocytosis, blood feeding, and P. falciparum infection induced dramatic shifts in granulocyte protein expression indicative of broad changes in cellular proliferation and innate immune response priming. Importantly, we identified a large number of hemocyte immune proteins that respond to blood feeding alone, suggesting that granulocytes may play an integral role in an anticipatory immune response prior to immune challenge.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Anopheles Gambiae (african Malaria Mosquito)

TISSUE(S): Whole Body

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: Dingyin Tao

LAB HEAD: Rhoel R. Dinglasan

PROVIDER: PXD001507 | Pride | 2016-09-26

REPOSITORIES: Pride

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Molecular Profiling of Phagocytic Immune Cells in Anopheles gambiae Reveals Integral Roles for Hemocytes in Mosquito Innate Immunity.

Smith Ryan C RC King Jonas G JG Tao Dingyin D Zeleznik Oana A OA Brando Clara C Thallinger Gerhard G GG Dinglasan Rhoel R RR

Molecular & cellular proteomics : MCP 20160913 11

The innate immune response is highly conserved across all eukaryotes and has been studied in great detail in several model organisms. Hemocytes, the primary immune cell population in mosquitoes, are important components of the mosquito innate immune response, yet critical aspects of their biology have remained uncharacterized. Using a novel method of enrichment, we isolated phagocytic granulocytes and quantified their proteomes by mass spectrometry. The data demonstrate that phagocytosis, blood- ...[more]

PMID: 27624304

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