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Proteomics method for secretome analysis of filamentous fungi

ABSTRACT: We describe a simple, plate-based method to analyze the secretomes of microorganisms growing on insoluble substrates that allows harsh sample preparation methods promoting desorption, and subsequent identification, of substrate-bound proteins, while minimizing contamination with non-secreted proteins from leaking or lysed cells. The method described here enables rapid large-scale comparative studies of the secretomes of filamentous fungi and other microorganisms growing on a variety of solid substrates, which eventually may have implications for our understanding of enzymatic lignocellulose degradation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Hypocrea Jecorina (strain Qm6a) (trichoderma Reesei)

SUBMITTER: Magnus Arntzen

LAB HEAD: Vincent Eijsink

PROVIDER: PXD001897 | Pride | 2015-10-26

REPOSITORIES: Pride

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A novel proteomics sample preparation method for secretome analysis of Hypocrea jecorina growing on insoluble substrates.

Bengtsson Oskar O Arntzen Magnus Ø MØ Mathiesen Geir G Skaugen Morten M Eijsink Vincent G H VGH

Journal of proteomics 20151023

Analysis of the secretomes of filamentous fungi growing on insoluble lignocellulosic substrates is of major current interest because of the industrial potential of secreted fungal enzymes. Importantly, such studies can help identifying key enzymes from a large arsenal of bioinformatically detected candidates in fungal genomes. We describe a simple, plate-based method to analyze the secretome of Hypocrea jecorina growing on insoluble substrates that allows harsh sample preparation methods promoti ...[more]

PMID: 26477388

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