Dataset Information

Multi-layered proteomics of EGFR signaling _Rab7 data

ABSTRACT: We immunoprecipitated Rab7-GFP wt and mutant upon EGF stimulation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: Chiara Francavilla

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD001996 | Pride | 2016-05-03

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20140703_QE3_UPLC11_KBE_SA_IP_CFR_Exp1_F1_01.raw	Raw
	20140703_QE3_UPLC11_KBE_SA_IP_CFR_Exp1_F1b_08.raw	Raw
	20140703_QE3_UPLC11_KBE_SA_IP_CFR_Exp1_F2_02.raw	Raw
	20140703_QE3_UPLC11_KBE_SA_IP_CFR_Exp1_F3_03.raw	Raw
	20140703_QE3_UPLC11_KBE_SA_IP_CFR_Exp1_F4_04.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Project description:Aberrant activation of Hedgehog (HH) signaling has been identified as a key etiologic factor of many human malignancies. Signal strength, target gene specificity, and oncogenic activity of HH signaling profoundly depend on interactions with other pathways such as epidermal growth factor receptor-mediated signaling which has been shown to cooperate with HH/GLI in basal cell carcinoma and pancreatic cancer. We demonstrate that the human medulloblastoma cell line Daoy possesses a fully inducible endogenous HH pathway. Treatment of Daoy cells with Sonic Hedgehog or Smoothened agonist induced expression of GLI1 protein and prevented processing of GLI3 to its repressor form. To study interactions between HH- and EGF-induced signaling in greater detail, time-resolved measurements were carried out and analyzed on the transcriptomic as well as proteomic level. Daoy cells responded to the co-treatment by downregulating GLI1, PTCH, and HHIP on the transcript level which was also seen when Amphiregulin (AREG) was used instead of EGF. The finding that EGFR signaling silences proteins acting as negative regulators of HH signaling is firstly described here as a novel crosstalk mechanism. Furthermore, combined EGFR/HH signaling maintains high GLI1 protein levels contrasting its downregulation on the transcript level. On the other hand, high level synergism was observed with respect to a strong and significant upregulation of numerous canonical EGF-targets with putative tumor-promoting properties such as MMP7, VEGFA, and IL-8. In conclusion, synergistic effects between EGFR and HH signaling can selectively induce a switch from a canonical HH/GLI profile to a modulated specific target gene profile pointing to more wide-spread, yet context-dependent, interactions between HH/GLI and growth factor receptor signaling in human malignancies. To study interactions between HH- and EGF-induced signaling, time-resolved measurements were carried out over a period of 24 h at 14 different time points after stimulation by EGF with and without additional stimulation by SHH. Furthermore, as a control, cells without any stimulation by EGF and SHH (control) and cells in the presents of SHH were analyzed. Overall, three biological replicates of 60 different treatment/timepoints were analyzed yielding 180 different samples.

Dataset Information

Multi-layered proteomics of EGFR signaling _Rab7 data

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets