Dataset Information

The proteome of native adult Müller cells from the murine retina

ABSTRACT: We set out to first establish and validate a novel rapid method of enriching adult Müller cells, which yields sufficient cell numbers for proteomic profiling while keeping cells viable and morphologically, as well as physiologically fully intact. From these preparations we collected a comprehensive data set of the proteome of native murine Müller cells. Since many Müller cell functions depend on cell-cell interactions occurring at the plasma membrane level, we additionally included a subcellular fractionation approach that enabled to discriminate proteins primarily residing in the cytosol or the nucleus from those allocated in the plasma membrane and thus generate a first time Müller cell surfaceome study. We consider these data a first important step to better understanding the complex crosstalk between various retinal cell types and Müller cells as key players in health and ultimately also under disease conditions.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Primary Cell, Muller Cell

DISEASE(S): Disease Free

SUBMITTER: Christine von Toerne

LAB HEAD: Stefanie Hauck

PROVIDER: PXD002247 | Pride | 2016-08-15

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	JBX7072A2MA4__002-positiv_B_0821_500000JBX7072A2MA4-40-259923.raw	Raw
	JBX7072A3MA4__003-positiv_A_0717_800000JBX7072A3MA4-40-259933.raw	Raw
	JBX7072A5MA4__005-negativ_D_0821_500000JBX7072A5MA4-40-259938.raw	Raw
	JBX7072A6MA4__006-negativ_C_0717_800000JBX7072A6MA4-40-259947.raw	Raw
	JBX7072_allMSMS.mgf	Mgf

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Publications

The Proteome of Native Adult Müller Glial Cells From Murine Retina.

Grosche Antje A Hauser Alexandra A Lepper Marlen Franziska MF Mayo Rebecca R von Toerne Christine C Merl-Pham Juliane J Hauck Stefanie M SM

Molecular & cellular proteomics : MCP 20150831 2

To date, the proteomic profiling of Müller cells, the dominant macroglia of the retina, has been hampered because of the absence of suitable enrichment methods. We established a novel protocol to isolate native, intact Müller cells from adult murine retinae at excellent purity which retain in situ morphology and are well suited for proteomic analyses. Two different strategies of sample preparation - an in StageTips (iST) and a subcellular fractionation approach including cell surface protein pro ...[more]

PMID: 26324419

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