Proteomics

Dataset Information

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MS proteomic datasets for "Development of methodology to investigate the surface SMALPome of mammalian cells".


ABSTRACT: In this study, mass spectrometry proteomics was used to investigate whether there are qualitative or quantitative differences in the mammalian plasma membrane proteins extracted with SMA compared to a detergent control. For this, cell surface proteins of 3T3L1 fibroblasts were biotinylated and extracted using either styrene maleic acid (SMA) or detergent. Following affinity pull-down of biotinylated proteins with NeutrAvidin beads, samples were analysed by nanoLC-MS. Here, we report for the first time, a global proteomics protocol for detection of a mammalian cell ‘SMALPome’, membrane proteins incorporated into SMA nanodiscs.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Fibroblast

SUBMITTER: Kerrie Morrison  

LAB HEAD: Paul Whitley

PROVIDER: PXD027735 | Pride | 2021-12-15

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
RIPA_protocol_1.msf Msf
RIPA_protocol_1.raw Raw
RIPA_protocol_2.msf Msf
RIPA_protocol_2.raw Raw
RIPA_protocol_3.msf Msf
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