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Root iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity

ABSTRACT: After long-term aluminum treatment, thereafter, we investigated Citrus root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Citrus Maxima (pomelo) (citrus Grandis) Citrus Sinensis (sweet Orange) (citrus Aurantium Var. Sinensis)

TISSUE(S): Plant Cell, Root

DISEASE(S): Disease Free

SUBMITTER: Lin-Tong Yang

LAB HEAD: Lisong Chen

PROVIDER: PXD002301 | Pride | 2017-10-26

REPOSITORIES: Pride

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			Action	DRS
	Citrus.zip	Other
	mgf_1.zip	Other
	wiff.zip	Other

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Publications

Root iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity.

Jiang Huan-Xin HX Yang Lin-Tong LT Qi Yi-Ping YP Lu Yi-Bin YB Huang Zeng-Rong ZR Chen Li-Song LS

BMC genomics 20151116

<h4>Background</h4>Limited information is available on aluminum (Al)-toxicity-responsive proteins in woody plant roots. Seedlings of 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) were treated for 18 weeks with nutrient solution containing 0 (control) or 1.2 mM AlCl3 · 6H2O (+Al). Thereafter, we investigated Citrus root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ). The aims of this work were to determine the molecular mechanisms of plants ...[more]

PMID: 26573913

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Phosphorus-mediated alleviation of aluminum toxicity revealed by iTRAQ technique in Citrus grandis roots

Project description:Citrus grandis seedlings were irrigated with nutrient solutions containing two levels of aluminium (Al; 0 mM and 1.2 mM AlCl3•6H2O) and two levels of phosphorus (P; 0 μM and 200 μM KH2PO4) combinations for 18 weeks, respectively. The proteome profile of Citrus root submitted to different P-Al interactions were investigated by using isobaric tags for relative and absolute quantification (iTRAQ).

2019-11-13 | PXD012534 | Pride

Comparative proteome of two citrus species differing in aluminum-tolerance in response to aluminum-toxicity revealed by iTRAQ

Project description:After long-term aluminum treatment, thereafter, we investigated leaves protein profiles of two citrus cultivars using isobaric tags for relative and absolute quantification (iTRAQ).

2018-10-24 | PXD002916 | Pride

Proteomic analysis of proteins related to rice grain chalkiness using iTRAQ based on a notched-belly mutant with white-belly

Project description:Grain chalkiness is a complex trait adversely affecting appearance and milling quality, and therefore has been one of principal targets for rice improvement. Eliminating chalkiness from rice has been a daunting task due to the complex interaction between genotype and environment and the lack of molecular markers. In addition, the molecular mechanisms underlying grain chalkiness formation are still imperfectly understood. We identified a notched-belly mutant (DY1102) with high percentage of white-belly, which only occurs in the bottom part proximal to the embryo. Using this mutant, a novel comparison system that can minimize the effect of genetic background and growing environment was developed. An iTRAQ-based comparative display of the proteins between the bottom chalky part and the upper translucent part of grains of DY1102 was performed. A total of 113 proteins responsible for chalkiness formation was identified. Among them, 70 proteins are up-regulated and 43 down-regulated. Approximately half of these differentially expressed proteins involved in central metabolic or regulatory pathways including carbohydrate metabolism (especially cell wall synthesis) and protein synthesis, folding and degradation, providing proteomic confirmation of the notion that chalkiness formation involves diverse but delicately regulated pathways. Protein metabolism was the most abundant category, accounting for 27.4% of the total differentially expressed proteins. In addition, down regulation of PDIL 2-3 and BiP was detected in the chalky tissue, indicating the important role of protein metabolism in grain chalkiness formation. Using this novel comparison system, our comprehensive survey of endosperm proteomics in the notched-belly mutant provides a valuable proteomic resource for the characterization of pathways contributing to chalkiness formation at molecular and biochemical levels.

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Project description:Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

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MS identification of the Cry proteins in Bacillus thuringiensis CT-43

Project description:In Bacillus thuringiensis CT-43, five insecticidal crystal proteins (ICPs, Cry protein) are encoded. We extracted the Cry proteins, ran the SDS PAGE (two Cry protein bands were observed), and tried to identify the composition of the two Cry protein bands in the SDS PAGE. The bioinformatics pipeline is described as follows: First, we converted the original mass spectrum files to the mgf file (peaks file), then the mgf files were searched against the Bacillusthuringiensis CT-43 protein database using Mascot (version 2.3.02). The search parameters were: i) trypsin was chosen as the enzyme with one missed cleavage allowed; ii) the fixed modifications of carbamidomethylation were set as Cys, and variable modifications of oxidation as Met; iii) peptide tolerance was set as 0.05 Da, and MS/MS tolerance was set as 0.1 Da. The peptide charge was set as Mr, and monoisotopic mass was chosen. An automatic decoy database search strategy was employed to estimate the false discovery rate (FDR). The FDR was calculated as the false positive matches divided by the total matches. In the final search results, the FDR was less than 1.5%.

2020-01-24 | PXD000425 | Pride

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The Hypervariable Amino-Terminus of P1 Protease Modulates Potyviral Replication and Host Defense Responses

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