Browse
Submit Data
Databases
API
Help

Dataset Information

243 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Root iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity

ABSTRACT: After long-term aluminum treatment, thereafter, we investigated Citrus root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Citrus Maxima (pomelo) (citrus Grandis) Citrus Sinensis (sweet Orange) (citrus Aurantium Var. Sinensis)

TISSUE(S): Plant Cell, Root

DISEASE(S): Disease Free

SUBMITTER: Lin-Tong Yang

LAB HEAD: Lisong Chen

PROVIDER: PXD002301 | Pride | 2017-10-26

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Citrus.zip	Other
	mgf_1.zip	Other
	wiff.zip	Other

Items per page:

1 - 3 of 3

Publications

Root iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity.

Jiang Huan-Xin HX Yang Lin-Tong LT Qi Yi-Ping YP Lu Yi-Bin YB Huang Zeng-Rong ZR Chen Li-Song LS

BMC genomics 20151116

<h4>Background</h4>Limited information is available on aluminum (Al)-toxicity-responsive proteins in woody plant roots. Seedlings of 'Xuegan' (Citrus sinensis) and 'Sour pummelo' (Citrus grandis) were treated for 18 weeks with nutrient solution containing 0 (control) or 1.2 mM AlCl3 · 6H2O (+Al). Thereafter, we investigated Citrus root protein profiles using isobaric tags for relative and absolute quantification (iTRAQ). The aims of this work were to determine the molecular mechanisms of plants ...[more]

PMID: 26573913

Similar Datasets

Phosphorus-mediated alleviation of aluminum toxicity revealed by iTRAQ technique in Citrus grandis roots

Project description:Citrus grandis seedlings were irrigated with nutrient solutions containing two levels of aluminium (Al; 0 mM and 1.2 mM AlCl3•6H2O) and two levels of phosphorus (P; 0 μM and 200 μM KH2PO4) combinations for 18 weeks, respectively. The proteome profile of Citrus root submitted to different P-Al interactions were investigated by using isobaric tags for relative and absolute quantification (iTRAQ).

2019-11-13 | PXD012534 | Pride

Comparative proteome of two citrus species differing in aluminum-tolerance in response to aluminum-toxicity revealed by iTRAQ

Project description:After long-term aluminum treatment, thereafter, we investigated leaves protein profiles of two citrus cultivars using isobaric tags for relative and absolute quantification (iTRAQ).

2018-10-24 | PXD002916 | Pride

Proteomic analysis of proteins related to rice grain chalkiness using iTRAQ based on a notched-belly mutant with white-belly

Project description:Grain chalkiness is a complex trait adversely affecting appearance and milling quality, and therefore has been one of principal targets for rice improvement. Eliminating chalkiness from rice has been a daunting task due to the complex interaction between genotype and environment and the lack of molecular markers. In addition, the molecular mechanisms underlying grain chalkiness formation are still imperfectly understood. We identified a notched-belly mutant (DY1102) with high percentage of white-belly, which only occurs in the bottom part proximal to the embryo. Using this mutant, a novel comparison system that can minimize the effect of genetic background and growing environment was developed. An iTRAQ-based comparative display of the proteins between the bottom chalky part and the upper translucent part of grains of DY1102 was performed. A total of 113 proteins responsible for chalkiness formation was identified. Among them, 70 proteins are up-regulated and 43 down-regulated. Approximately half of these differentially expressed proteins involved in central metabolic or regulatory pathways including carbohydrate metabolism (especially cell wall synthesis) and protein synthesis, folding and degradation, providing proteomic confirmation of the notion that chalkiness formation involves diverse but delicately regulated pathways. Protein metabolism was the most abundant category, accounting for 27.4% of the total differentially expressed proteins. In addition, down regulation of PDIL 2-3 and BiP was detected in the chalky tissue, indicating the important role of protein metabolism in grain chalkiness formation. Using this novel comparison system, our comprehensive survey of endosperm proteomics in the notched-belly mutant provides a valuable proteomic resource for the characterization of pathways contributing to chalkiness formation at molecular and biochemical levels.

2014-06-17 | PXD001030 | Pride

Ctenopharyngodon idella iTRAQ - Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

Project description:Understanding the Immune Mechanisms of Vibrio mimicus targeted epitopes gene vaccine by iTRAQ Technology

2018-12-18 | PXD012011 | Pride

MS identification of the Cry proteins in Bacillus thuringiensis CT-43

Project description:In Bacillus thuringiensis CT-43, five insecticidal crystal proteins (ICPs, Cry protein) are encoded. We extracted the Cry proteins, ran the SDS PAGE (two Cry protein bands were observed), and tried to identify the composition of the two Cry protein bands in the SDS PAGE. The bioinformatics pipeline is described as follows: First, we converted the original mass spectrum files to the mgf file (peaks file), then the mgf files were searched against the Bacillusthuringiensis CT-43 protein database using Mascot (version 2.3.02). The search parameters were: i) trypsin was chosen as the enzyme with one missed cleavage allowed; ii) the fixed modifications of carbamidomethylation were set as Cys, and variable modifications of oxidation as Met; iii) peptide tolerance was set as 0.05 Da, and MS/MS tolerance was set as 0.1 Da. The peptide charge was set as Mr, and monoisotopic mass was chosen. An automatic decoy database search strategy was employed to estimate the false discovery rate (FDR). The FDR was calculated as the false positive matches divided by the total matches. In the final search results, the FDR was less than 1.5%.

2020-01-24 | PXD000425 | Pride

Proteomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in Synechocystis sp. PCC 6803

Project description:Using an engineered 3-HP producing SM strain, in this project, the cellular responses to 3-HP internally produced were first determined using a quantitative iTRAQ-LC-MS/MS proteomics approach. A total of 2,264 unique proteins were identified, which represented about 63% of all predicted protein in Synechocystis in the proteomic analysis of the 3-HP producing strain SM and wild type. Among all identified proteins, 204 proteins were found up- regulated and 123 proteins were found down-regulated, respectively. The proteins related to oxidative phosphorylation, photosynthesis, ribosome, central carbon metabolism, two-component systems and ABC-type transporters were up-regulated. The results suggested that the supply of ATP and NADPH was increased significantly, and the precursor malonyl-CoA and acetyl-CoA may also be supplemented when 3-HP was produced at a high level in Synechocystis.

2016-09-26 | PXD004974 | Pride

The Hypervariable Amino-Terminus of P1 Protease Modulates Potyviral Replication and Host Defense Responses

Project description:The replication of many RNA viruses involves the translation of polyproteins, whose processing by endopeptidases is a critical step for the release of functional subunits. P1 is the first protease encoded in plant potyvirus genomes; once activated by an as-yet-unknown host factor, it acts in cis on its own C-terminal end, hydrolyzing the P1-HCPro junction. Earlier research suggests that P1 cooperates with HCPro to inhibit host RNA silencing defenses. Using Plum pox virus as a model, we show that although P1 does not have a major direct role in RNA silencing suppression, it can indeed modulate HCPro function by its self-cleavage activity. To study P1 protease regulation, we used bioinformatic analysis and in vitro activity experiments to map the core C-terminal catalytic domain. We present evidence that the hypervariable region that precedes the protease domain is predicted as intrinsically disordered, and that it behaves as a negative regulator of P1 proteolytic activity in in vitro cleavage assays. In viral infections, removal of the P1 protease antagonistic regulator is associated with greater symptom severity, induction of salicylate-dependent pathogenesis-related proteins, and reduced viral loads. We suggest that fine modulation of a viral protease activity has evolved to keep viral amplification below host-detrimental levels, and thus to maintain higher long-term replicative capacity.

2020-08-11 | PXD017769 | Pride

HEK293 cells LC-MSMS - XBP1 mitigates aminoglycoside-induced endoplasmic reticulum stress and neuronal cell death

Project description:The iTRAQ 4-plex experiment was carried out with 2 biological replicates of untreated HEK cells (114 and 116 label) and 2 biological replicates of HEK cells treated with geneticin (16 µM) for 32 h (115 and 117 label).

2015-08-10 | PXD000933 | Pride

The iTRAQ-based proteome profile was also identified in the mutant and WT to provide a deeper insight into post-transcriptional modifications

Project description:The iTRAQ-based proteome profile was also identified in the mutant and WT to provide a deeper insight into post-transcriptional modifications.

2020-05-27 | PXD017197 | Pride

Proteomic Analysis of dental pulp stem cell-derived exosomes

Project description:In order to examine the proteomics of exosomes derived from DPSCs under hypoxia (Hypo-Exos) and the mechanism underlying the angiogenic effect of DPSC-Exos, iTRAQ-based proteomics analysis and bioinformatic analysis were performed to investigate proteome profile differences between exosomes derived from DPSCs under normoxia (Nor-Exos) and Hypo-Exos.7114 peptides and 1792 proteins were identified from the Hypo-Exos samples with a 1% FDR. Compared with the control group, 39 proteins were upregulated and 40 proteins were downregulated in Hypo-Exos.

2022-05-20 | PXD032719 | Pride