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A novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics

ABSTRACT: Tandem-affinity purification mass spectrometry using the streptavidin-hemagglutinin (SH)-tag has been successfully employed to map signaling networks in several large-scale studies. However its application has so far been restricted to the small number of Flp/FRT-recombination competent cell lines. We present pRSHIC, a novel retroviral, doxycycline-inducible Tet-On vector system suitable for expression of SH-tagged target proteins in a wide range of human and mouse cell systems. The additional feature of concomitant reporter fluorophore expression makes pRSHIC a valuable tool for a diverse set of phenotypic analyses beyond TAP-MS experiments. The dataset demonstrates the application of pRSHIC for TAP-MS analysis of two showcase bait proteins involved in cancer cell proliferation as well as cell death induction and identified novel high- confidence interacting proteins with possible pharmacological intervention potential.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Richard Kumaran Kandasamy

LAB HEAD: Giulio Superti-Furga

PROVIDER: PXD002855 | Pride | 2016-01-05

REPOSITORIES: Pride

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			Action	DRS
	M484-C01-C2796-P5902-1.dat	Other
	M484-C01-C2796-P5902-1.mgf	Mgf
	M484-C01-C2796-P5902-1.mzid.gz	Mzid
	M484-C01-C2796-P5902-1.pride.mgf.gz	Mgf
	M484-C01-C2796-P5902-1.pride.mztab.gz	Mztab

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A novel inducible retroviral expression system for StrepHA-tandem affinity purification mass-spectrometry-based proteomics

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