Dataset Information

Two unrelated 8-vinyl reductases ensure production of mature chlorophylls in Acaryochloris marina

ABSTRACT: The aim of this project is to identify, by bottom-up proteomic analysis, one or both representatives of two classes of 8-vinyl reductase in an Acaryochloris marina cell lysate. This enzyme is responsible for the conversion of the C8 vinyl group in the chlorophyll precursor Mg-divinyl-protochlorophyllide to ethyl as part of the chlorphyll biosynthesis pathway. Both putative 8VRs, NmrA and FrhB were detected.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Acaryochloris Marina (strain Mbic 11017)

TISSUE(S): Photosynthetic Cell

DISEASE(S): Disease Free

SUBMITTER: Philip Jackson

LAB HEAD: Prof. C Neil Hunter, FRS

PROVIDER: PXD003139 | Pride | 2020-05-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20151016_Acaryo_01.mgf	Mgf
	20151016_Acaryo_01.raw	Raw
	20151016_Acaryo_01_02_merged.dat	Other
	20151016_Acaryo_01_02_merged.dat-pride.pride.mgf.gz	Mgf
	20151016_Acaryo_01_02_merged.dat-pride.pride.mztab.gz	Mztab

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Publications

Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen Guangyu E GE Hitchcock Andrew A Jackson Philip J PJ Chaudhuri Roy R RR Dickman Mark J MJ Hunter C Neil CN Canniffe Daniel P DP

Journal of bacteriology 20160414 9

<h4>Unlabelled</h4>The major photopigment of the cyanobacterium Acaryochloris marina is chlorophyll d, while its direct biosynthetic precursor, chlorophyll a, is also present in the cell. These pigments, along with the majority of chlorophylls utilized by oxygenic phototrophs, carry an ethyl group at the C-8 position of the molecule, having undergone reduction of a vinyl group during biosynthesis. Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known t ...[more]

PMID: 26903415

Similar Datasets

Project description:UnlabelledThe major photopigment of the cyanobacterium Acaryochloris marina is chlorophyll d, while its direct biosynthetic precursor, chlorophyll a, is also present in the cell. These pigments, along with the majority of chlorophylls utilized by oxygenic phototrophs, carry an ethyl group at the C-8 position of the molecule, having undergone reduction of a vinyl group during biosynthesis. Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB. The genome of Acaryochloris marina contains open reading frames (ORFs) encoding proteins displaying high sequence similarity to BciA or BciB, although they are annotated as genes involved in transcriptional control (nmrA) and methanogenesis (frhB), respectively. These genes were introduced into an 8-vinyl chlorophyll a-producing ΔbciB strain of Synechocystis sp. strain PCC 6803, and both were shown to restore synthesis of the pigment with an ethyl group at C-8, demonstrating their activities as 8-vinyl reductases. We propose that nmrA and frhB be reassigned as bciA and bciB, respectively; transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.ImportanceThe cyanobacterium Acaryochloris marina is the best-studied phototrophic organism that uses chlorophyll d for photosynthesis. Unique among cyanobacteria sequenced to date, its genome contains ORFs encoding two unrelated enzymes that catalyze the reduction of the C-8 vinyl group of a precursor molecule to an ethyl group. Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.