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Effect of TPA on focal adhesion composition

ABSTRACT: Effect of a tumor promoter TPA on collagen IV based focal adhesions from CHO cells expressing human integrin alpha1 with activating mutation E317A

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: Pekka Rappu

LAB HEAD: Jyrki Heino

PROVIDER: PXD003153 | Pride | 2016-07-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	130306Pekka_Export_peptide_norm2all.csv	Csv
	130306Pekka_Export_protein_norm2all.csv	Csv
	130306_PekkaR_EtOH1.raw	Raw
	130306_PekkaR_EtOH2.raw	Raw
	130306_PekkaR_EtOH3.raw	Raw

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Publications

Tumor promoter PMA enhances kindlin-2 and decreases vimentin recruitment into cell adhesion sites.

Salmela Maria M Rappu Pekka P Lilja Johanna J Niskanen Henri H Taipalus Elina E Jokinen Johanna J Heino Jyrki J

The international journal of biochemistry & cell biology 20160629

Phorbol diester PMA (phorbol 12-myristate 13-acetate) is a well-known promoter of tumor progression. PMA also regulates cell adhesion by several mechanisms including conformational activation of integrins and integrin clustering. Here, PMA was shown to induce lamellipodia formation and reorganization of the adhesion sites as well as actin and vimentin filaments independently of integrin preactivation. To further analyze the mechanism of PMA action, the protein composition in the α1β1 integrin/co ...[more]

PMID: 27373681

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