Proteomics

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An up to date workflow for plant (phospho)proteomics

ABSTRACT: Protein phosphorylation is one of the most common post-translational modifications (PTMs), which can regulate protein activity and localization, as well as protein–protein interactions in numerous cellular processes. Phosphopeptide enrichment techniques enabled plant researchers to acquire insight in phosphorylation-controlled signaling networks in various plant species. However, most phosphoproteome analyses of plant samples still involve stable isotope labeling, peptide fractionation, and demand lots of mass spectrometry (MS) time. Here, we present a simple workflow to probe, map and catalogue plant phosphoproteomes, requiring low starting materials, no labeling, no fractionation, and limited analysis time. Following optimization of the different experimental steps on Arabidopsis material, we transferred our workflow to maize, a major monocot crop, to study stress signaling upon drought.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) Zea Mays (maize)

TISSUE(S): Plant Cell, Root, Leaf

SUBMITTER: Elisabeth Stes  

LAB HEAD: Ive De Smet

PROVIDER: PXD003634 | Pride | 2016-09-26

REPOSITORIES: Pride

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E07244_1p_4479_maize_1p.raw Raw
E07245_2p_4479_maize_2p.raw Raw
E07246_1p_4479_maize_3p.raw Raw
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Publications

Up-to-Date Workflow for Plant (Phospho)proteomics Identifies Differential Drought-Responsive Phosphorylation Events in Maize Leaves.

Vu Lam Dai LD   Stes Elisabeth E   Van Bel Michiel M   Nelissen Hilde H   Maddelein Davy D   Inzé Dirk D   Coppens Frederik F   Martens Lennart L   Gevaert Kris K   De Smet Ive I  

Journal of proteome research 20161004 12

Protein phosphorylation is one of the most common post-translational modifications (PTMs), which can regulate protein activity and localization as well as protein-protein interactions in numerous cellular processes. Phosphopeptide enrichment techniques enable plant researchers to acquire insight into phosphorylation-controlled signaling networks in various plant species. Most phosphoproteome analyses of plant samples still involve stable isotope labeling, peptide fractionation, and demand a lot  ...[more]

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