Proteomics

Dataset Information

0

Dynamic of the Grb2 interactome in primary cells by DIA-MS


ABSTRACT: We applied AP-SWATH to quantify Grb2 interaction dynamics in primary T cells with a high level of reproducibility and accuracy. We concluded that AP-SWATH is a robust approach to quantify signaling dynamics in primary cells and could be further expanded to additional hub proteins using a range of cell and tissues types.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen, T Cell, Lymph Node, Thymus

SUBMITTER: Etienne Caron  

LAB HEAD: Matthias Gstaiger

PROVIDER: PXD003972 | Pride | 2017-03-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
E1601101045_feature_alignment_requant.tsv.gz Tabular
SpecLib_WTOST.TraML Other
SpecLib_WTOST_openswath.tsv Tabular
annotationPeriphery.txt Txt
annotationThymus.txt Txt
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Publications


Spatiotemporal organization of protein interactions in cell signaling is a fundamental process that drives cellular functions. Given differential protein expression across tissues and developmental stages, the architecture and dynamics of signaling interaction proteomes is, likely, highly context dependent. However, current interaction information has been almost exclusively obtained from transformed cells. In this study, we applied an advanced and robust workflow combining mouse genetics and af  ...[more]

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