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Comparing search algorithms - Visualizing and comparing results of different peptide identification methods

ABSTRACT: An approach to compare different peptide identification methods

INSTRUMENT(S): amaZon Speed ETD, Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Yassene Mohammed

LAB HEAD: Yassene Mohammed

PROVIDER: PXD005118 | Pride | 2017-01-02

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	091201.LC4.IT4.EC.S01215.Ecoli_1-E6_01_173.mzXML	Mzxml
	091201.LC4.IT4.EC.S01215.Ecoli_1-E6_01_173.tandem.pep.xml	Pepxml
	q2151259c_.mzXML	Mzxml
	q2151259c_.pep.xml	Pepxml

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Visualizing and comparing results of different peptide identification methods.

Mohammed Yassene Y Palmblad Magnus M

Briefings in bioinformatics 20180301 2

In mass spectrometry-based proteomics, peptides are typically identified from tandem mass spectra using spectrum comparison. A sequence search engine compares experimentally obtained spectra with those predicted from protein sequences, applying enzyme cleavage and fragmentation rules. To this, there are two main alternatives: spectral libraries and de novo sequencing. The former compares measured spectra with a collection of previously acquired and identified spectra in a library. De novo attemp ...[more]

PMID: 28011752

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