Dataset Information

Proteomic comparison of tissue engineered tendon constructs derived young and old cells

ABSTRACT: Tendon injuries can occur due to sports related incidents, as a result of trauma to overuse or during disease or ageing. Tissue engineering can offer great potential in the treatment of a tendon injury. The preferred cell source is autologous in order to reduce immune response in the treated individual. However, in elderly patients age-related changes in metabolism of the implanted cells may affect results of such therapy. In this study the effect of donor age on synthetic activity of cells used for creation of tendon tissue-engineered constructs was investigated by using label-free proteomic comparison.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Tendon Cell, Tendon

SUBMITTER: Yalda Ashraf Kharaz

LAB HEAD: Dr Mandy Peffers

PROVIDER: PXD005322 | Pride | 2018-10-26

REPOSITORIES: Pride

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Publications

Donor age affects proteome composition of tenocyte-derived engineered tendon.

Turlo Agnieszka J AJ Ashraf Kharaz Yalda Y Clegg Peter D PD Anderson James J Peffers Mandy J MJ

BMC biotechnology 20180116 1

<h4>Background</h4>The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce immune response in the treated individual. However, in elderly patients age-related changes in synthetic activity of the implanted cells and subsequent alterations in tissue protein content may affect therapeutic outcomes. In this study we investigated the effect of donor ...[more]

PMID: 29338716

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