Dataset Information

Grapevine Leaf proteome - Overexpression of a stress-responsive U-box protein gene VaPUB affects the accumulation of resistance related proteins in Vitis vinifera 'Thompson Seedless'

ABSTRACT: To investigate differentially accumulated proteins caused by transgenic events, comparative proteomic analysis using two-dimensional polyacrylamide gel electrophoresis was employed for Vitis vinifera ‘Thompson seedless’ leaves and transgenic Vitis vinifera ‘Thompson seedless’ leaves. Obtained proteins were digested with trypsin and subjected to MALDI-TOF-MS/MS analysis. According to annotations and a Blast search, proteins analyzed by MALDI-TOF/TOF were classified into 4 functional categories: carbon and energy metabolism, oxidation reaction, protein metabolism and resistance.

INSTRUMENT(S): 5800 TOF/TOF

ORGANISM(S): Vitis Vinifera (grape)

TISSUE(S): Leaf

SUBMITTER: Li JIAO

LAB HEAD: Li JIAO

PROVIDER: PXD005570 | Pride | 2017-01-04

REPOSITORIES: Pride

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Overexpression of a stress-responsive U-box protein gene VaPUB affects the accumulation of resistance related proteins in Vitis vinifera 'Thompson Seedless'.

Jiao Li L Zhang Yali Y Lu Jiang J

Plant physiology and biochemistry : PPB 20161225

Many U-box proteins have been identified and characterized as important factors against environmental stresses such as chilling, heat, salinity and pathogen attack in plant. Our previous research reported the cloning of a novel U-box protein gene VaPUB from Vitis amurensis 'Zuoshanyi' grape and suggested a function of it in related to cold stress in the model plant Arabidopsis system. In this study, the role of VaPUB in response to biotic and abiotic stress was further analyzed in the homologous ...[more]

PMID: 28039816

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