Dataset Information

BHK-21 cell iTRAQ - Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells

ABSTRACT: Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, DTMUV can also infect geese, chickens, house sparrows and replicate in in the brain, spleen, liver and kidneys of BALB/c mice, which poses public health concerns. So developing vaccines to combat DTMUV becomes urgent. Baby hamster kidney cell line (BHK-21) is usually employed to produce veterinary vaccine and DTMUV as well as other flaviviruses can be propagated in BHK-21 cells. However, information about mammalian host cell responses to DTMUV infection is limited. In this study, LC–MS/MS coupled to iTRAQ labeling was used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells. We identified 192 differentially expressed cellular proteins including 11 upregulated proteins and 8 downregulated at 24 hpostinfection; 25 upregulated proteins and 151 downregulated at 48 h postinfection. Some of these proteins were involved in viral infection.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Cell Culture

SUBMITTER: Xin Sun

LAB HEAD: Meilin Jin

PROVIDER: PXD005731 | Pride | 2017-05-23

REPOSITORIES: Pride

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			Action	DRS
	HZKDCSi1020150125.wiff	Wiff
	HZKDCSi1020150125.wiff.scan	Wiff
	HZKDCSi120150125.wiff	Wiff
	HZKDCSi120150125.wiff.scan	Wiff
	HZKDCSi220150125.wiff	Wiff

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Publications

Proteome analysis of Duck Tembusu virus (DTMUV)-infected BHK-21 cells.

Sun Xin X Wang Shengyu S Lin Xian X Zhao Lianzhong L Zhang Dan D Yi Chenyang C Sun Xiaomei X Chen Huanchun H Jin Meilin M

Proteomics 20170601 12

Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that has caused huge economic losses to the duck industry in China since 2010. Moreover, the infection has spread rapidly, posing a potential public health concern. In this study, iTRAQ approach was first used to quantitatively identify differentially expressed cellular proteins in DTMUV-infected BHK-21 cells which are usually employed to produce veterinary vaccines for DTMUV, as well as other flaviviruses by serial passage. We ...[more]

PMID: 28516729

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