Dataset Information

Proteome differences between sporulated oocysts of ToxoDB#9 and Type II Toxoplasma gondii

ABSTRACT: Understanding of protein expression difference in the same stage of T.gondii among different genotypes will facilitate the elucidation of genotypic divergence among the T.gondii strains. A 4-plex iTRAQ (isobaric tag for relative and absolute quantitation) based LC-MS/MS approach was employed to survey the differentially expressed proteins of sporulated oocysts between ToxoDB#1 (PRU) strain and ToxoDB#9 (PYS) strain for the first time.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii

SUBMITTER: Ze-Xiang Wang

LAB HEAD: Xing-Quan Zhu

PROVIDER: PXD005928 | Pride | 2018-10-26

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	20150310ToxoGondii_Itraq4_01.raw	Raw
	20150310ToxoGondii_Itraq4_02.raw	Raw
	20150310ToxoGondii_Itraq4_03.raw	Raw
	20150310ToxoGondii_Itraq4_04.raw	Raw
	20150310ToxoGondii_Itraq4_05.raw	Raw

Items per page:

1 - 5 of 52

Publications

Comparative proteomic analysis of virulent and avirulent strains of Toxoplasma gondii reveals strain-specific patterns.

Zhou Dong-Hui DH Wang Ze-Xiang ZX Zhou Chun-Xue CX He Shuai S Elsheikha Hany M HM Zhu Xing-Quan XQ

Oncotarget 20170707 46

Research exploring the proteome of Toxoplasma gondii oocysts has gained momentum over the past few years. However, little is known about the oocyst's protein repertoires that contribute to differential virulence among T. gondii strains. Here, we used isobaric tag for relative and absolute quantitation-based proteomic analysis of oocysts of two T. gondii strains exhibiting the virulent PYS (ToxoDB#9) phenotype versus the less virulent PRU (Type II, ToxoDB#1) phenotype. Our ai ...[more]

PMID: 29113319

Similar Datasets

Project description:Phosphorylation is a regulatory mechanism by which intracellular apicomplexan parasites control the process of invading and modifying host cells. However, the sporulated oocysts’ phosphoprotein repertoires between virulent and avirulent strains of Toxoplasma gondii and the underlying mechanisms contributing to virulence is still a mystery. A quantitative analysis of the sporulated oocysts’ phosphoproteome profile of T. gondii virulent and avirulent strains belonging to different genotypes was conducted to reveal phosphoprotein expression patterns that contribute to the virulence of a particular phenotype. Phosphopeptides from the sporulated oocysts of the virulent strain PYS (Chinese ToxoDB#9) and the avirulent strain type II (PRU strain) were enriched by titanium dioxide (TiO2) affinity chromatography and quantified using iBT technology. A total of 10,645 unique phosphopeptides, 8,181 nonredundant phosphorylation sites and 2,792 phosphoproteins were identified. The quantitative analysis identified 4,129 differentially expressed phosphopeptides (DEPs) between sporulated oocysts of the PYS strain and PRU strain (|log1.5 fold change| > 1 and P < 0.05), including 2,485 upregulated and 1,644 downregulated phosphopeptides. Motif analysis identified 24 motifs from the upregulated phosphorylated peptides including 22 serine motifs and two threonine motifs (TPE and TP), and 15 motifs from the downregulated phosphorylated peptides including 12 serine motifs and three threonine motifs (TP, RxxT and KxxT) in PYS strain when comparing PYS/PRU. Several kinases were consistent with motifs of overrepresented phosphopeptides, such as PKA, PKG, CKII, IKK, MAPK, EGFR, INSR, Jak, Syk, Src, Ab1. GO analysis, KEGG pathway analysis and STRING analysis revealed differentially expressed phosphoproteins (DEPs) exhibiting significant functional properties. Kinase associated network analysis showed that AGC kinase had the greatest connected peptides. Our findings reveal significant difference in phosphopeptides of sporulated oocysts between virulent and avirulent T. gondii strains, which provides solid foundation for further exploring the mechanisms contributing to the virulence of T. gondii.

Dataset Information

Proteome differences between sporulated oocysts of ToxoDB#9 and Type II Toxoplasma gondii

Dataset's files

Publications

Comparative proteomic analysis of virulent and avirulent strains of <i>Toxoplasma gondii</i> reveals strain-specific patterns.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets