Dataset Information

Bile Salts Enhance the Susceptibility of the Allergenic Lipid Transfer Protein from Peach, Pru p 3 to in vitro gastrointestinal digestion

ABSTRACT: Sensitisation to the lipid transfer protein Pru p 3 is associated with severe allergic reactions to peach, the proteins stability being thought to play a role in its allergenicity. Lipid binding increases susceptibility of Pru p 3 to digestion and so the impact of bile salts on the in vitro gastrointestinal digestibility of Pru p 3 was investigated and digestion products mapped by SDS PAGE and mass spectrometry. Bile salts enhanced the digestibility of Prup3 resulting in an ensemble of around 100 peptides spanning the proteins sequence which were linked by disulphide bonds into structures of ~5-6kDa. IgE binding studies with a serum panel from peach allergic subjects showed digestion reduced, but did not abolish, the IgE reactivity of Pru p 3. These data show the importance of including bile salts in in vitro digestion systems and emphasise the need to profile of digestion in a manner that allows identification of immunologically relevant disulphide-linked peptide aggregates.

INSTRUMENT(S): Xevo G2-XS QTof

ORGANISM(S): Prunus Persica

SUBMITTER: Matthew Daly

LAB HEAD: Clare Mills

PROVIDER: PXD037801 | Pride | 2023-10-24

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Prup3_GD2.5_DD__Peptide_measurements_databasedUpdated.csv	Csv
	Prup3_GD2.5_DD__Protein_measurements_databasedUpdated.csv	Csv
	Prup3_GD2.5_reduced_DD__Peptide_measurements_databasedUpdated.csv	Csv
	Prup3_GD2.5_reduced_DD__Protein_measurements_databasedUpdated.csv	Csv
	mbmhrkw2_070521_1to10_3-F1.raw.zip	Raw

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Publications

Bile salts enhance the susceptibility of the peach allergenic lipid transfer protein, Pru p 3, to in vitro gastrointestinal proteolysis.

Wang Kai K Gali-Moya Judit J Ruano-Zaragoza Maria M Cain Kathleen K D'Auria Giovanni G Daly Matthew M Barran Perdita P Crevel René R Mills E N Clare ENC

Scientific reports 20230913 1

Sensitisation to the lipid transfer protein Pru p 3 is associated with severe allergic reactions to peach, the proteins stability being thought to play a role in its allergenicity. Lipid binding increases susceptibility of Pru p 3 to digestion and so the impact of bile salts on the in vitro gastrointestinal digestibility of Pru p 3 was investigated and digestion products mapped by SDS-PAGE and mass spectrometry. Bile salts enhanced the digestibility of Pru p 3 resulting in an ensemble of around ...[more]

PMID: 37704681

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