Dataset Information

RNA polymerase II Immunoprecipitation from Arabidopsis LC-MSMS

ABSTRACT: RNA polymerase II (Pol II) was immunoprecipitated from Arabidopsis thaliana seedlings, using antibodies that specifically recognize: 1) C’ terminal Domain (CTD), 2) CTD Serine 5 Phosphorylation and 3) CTD Serine 2 Phosphorylation. Proteins that co-immunoprecipitate with different Pol II pools were analysed.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seedling

SUBMITTER: Nicolas Nalpas

LAB HEAD: Sascha Laubinger

PROVIDER: PXD006004 | Pride | 2018-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20161214_CO_0681SaLa_R13.raw	Raw
	20161214_CO_0681SaLa_R14.raw	Raw
	20161214_CO_0681SaLa_R15.raw	Raw
	20170110_CO_0681SaLa_R18.raw	Raw
	20170110_CO_0681SaLa_R19.raw	Raw

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Publications

Arabidopsis RNA processing factor SERRATE regulates the transcription of intronless genes.

Speth Corinna C Szabo Emese Xochitl EX Martinho Claudia C Collani Silvio S Zur Oven-Krockhaus Sven S Richter Sandra S Droste-Borel Irina I Macek Boris B Stierhof York-Dieter YD Schmid Markus M Liu Chang C Laubinger Sascha S

eLife 20180828

Intron splicing increases proteome complexity, promotes RNA stability, and enhances transcription. However, introns and the concomitant need for splicing extend the time required for gene expression and can cause an undesirable delay in the activation of genes. Here, we show that the plant microRNA processing factor SERRATE (SE) plays an unexpected and pivotal role in the regulation of intronless genes. Arabidopsis SE associated with more than 1000, mainly intronless, genes in a transcription-de ...[more]

PMID: 30152752

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