Proteomics

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Predictive biomarkers of sperm quality in sturgeon spermatozoa

ABSTRACT: In this study, we used the iTRAQ proteomics approach to perform proteomic profiling of spermatozoa associated with sperm quality in sturgeons. The results showed 291 and 359 differentially expressed proteins in A. baerii and A. schrenckii, respectively, of which 72 were common to both species and all were upregulated in high quality compared with low quality samples. The differentially expressed proteins were mainly categorized into the generation of precursor metabolites and energy and oxidation, and they were localized to the mitochondria. Three distinguishing pathways, Arginine and proline metabolism, Pyruvate metabolism and the Citrate cycle (TCA cycle) were found to play an important role in energy metabolism, and some substrates could be used in the sperm medium for storage and cryopreservation.

INSTRUMENT(S): TripleTOF 5600+

ORGANISM(S): Acipenser Baerii X Acipenser Schrenckii Acipenser

TISSUE(S): Germ Cell

SUBMITTER: jingjing huang  

LAB HEAD: Ping Li

PROVIDER: PXD006108 | Pride | 2017-10-31

REPOSITORIES: Pride

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Xunyu_AB_4iTRAQ.RAW.zip Raw
Xunyu_AB_4iTRAQ.pride.mgf.gz Mgf
Xunyu_AB_4iTRAQ.pride.mztab.gz Mztab
Xunyu_AB_4iTRAQ.xml.gz Xml
Xunyu_ASK_4iTRAQ.RAW.zip Raw
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Publications

Variability in the protein profiles in spermatozoa of two sturgeon species.

Li Ping P   Guo Wei W   Yue Huamei H   Li Chuangju C   Du Hao H   Qiao Xinmei X   Liu Zhigang Z   Zhou Qiong Q   Wei Qiwei Q  

PloS one 20171027 10

Conventional sperm analysis (i.e., motility and fertility) has been used to evaluate sperm quality. Understanding the quality of sperm on the molecular level in the sturgeons, Acipenser baerii and A. schrenckii, is essential for the improvement of the conservation of genetic resources and farming performance. In this study, we used the iTRAQ proteomics approach to perform proteomic profiling of spermatozoa associated with sperm quality in sturgeons (Data are available via ProteomeXchange with id  ...[more]

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